IN VITRO CULTIVATION OF PLASMODIUM SPECIES AND DETECTION OF ANTIPLASMODIAL ACTIVITY OF SOME DRUGS ON THE PARASITES
IN VITRO CULTIVATION OF PLASMODIUM SPECIES AND DETECTION OF ANTIPLASMODIAL ACTIVITY OF SOME DRUGS ON THE PARASITES
No Thumbnail Available
Date
2018-11
Authors
ADAMU, IYANG COMFORT
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Several reports have conducted resistance of Plasmodium to antimalarial in use. Successful cultivation and susceptibility will help to identify effectiveness of current antimalarial in use. The study was aimed at cultivating Plasmodium falciparum in-vitro and determination of the antiplasmodial activity of some drugs against malaria parasites. In this study, Blood samples were obtained from antenatal women attending some hospital in Zaria. Thin blood films were prepared stained using Giemsa stain and Acridine Orange stain. The films were observed microscopically using bright field microscope and fluorescence microscope (T6200 MIJEI). Antiplasmodial activity of some drugs to Plasmodium falciparum was carried out in-vitro. The prevalence of Plasmodium infection among antenatal patients was 27% and 53% by Giemsa and Acridine Orange Fluorescence microscopy. Factors found to be associated with the disease were lack of awareness of the aetiology of malaria (OR= 1.026; 95%; p-value= 1.000 CI= 0.585-1.799) and type of drainage (OR= 1.192; 95%; p- value= 0.129; CI= 0.456-3.113). The modified Jatropha medium supported the growth of the red blood cells with parasite density of 6500-12000as compared to 8000-14000 parasites/μl for those grown in Rosewell Park Memorial Institute medium. Results obtained from the in-vitro cultivation of P. falciparum with some drugs showed that chloroquine was able to reduce the parasite density from 7000 parasites/μl and 7500 parasites/μl with Rosewell Park Memorial Institute and Jatropha plant media at the 24th and 12th hour respectively compare to Artesunate, Quinine and Sulphadoxine + Pyrimethamine. There is need to further improve the antiplasmodial activity of some drugs to Plasmodium parasite in order to establish a malaria free nation.
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE MASTER OF SCIENCE DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
IN VITRO CULTIVATION,, PLASMODIUM SPECIES,, DETECTION OF ANTIPLASMODIAL ACTIVITY,, SOME DRUGS ON THE PARASITES