EFFECT OF ORAL ADMINISTRATION OF ALUMINIUM CHLORIDE ON THE HIPPOCAMPUS (BRAIN) OF WISTAR RATS
EFFECT OF ORAL ADMINISTRATION OF ALUMINIUM CHLORIDE ON THE HIPPOCAMPUS (BRAIN) OF WISTAR RATS
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Date
2008-02
Authors
BURAIMOH, ADEBAYO ADEKUNLE
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Abstract
The study of the effect of oral administration of aluminium chloride on the
Hippocampus of wistar rats was carried out in order to ascertain whether the small
daily amount of aluminium that gain access to the body produce any accumulation
damage to the hippocampus and if there is any association between drinking water
containing aluminium and brain damage. This investigation was carried out using 50
female adult wistar rats. The animals were divided into five groups; 10 rats per group.
Stock solution of aluminium chloride was prepared (2g/l or 2mg/ml).Different
concentrations of aluminium was administered to different groups orally. Group I was
control, while Groups II– V were given 2.3mg/kg(0.4mg),5.7mg/kg(1mg),11.4mg/kg
(2mg),and17.1mg/kg (3mg) respectively with an average weight of between 150-200g
for duration of twelve (12) weeks. Blood was collected from the tails of the rats for
haematological indices. The animals were sacrificed using chloroform and then the
brain tissues were fixed immediately in Bouin’s fluid. The hippocampus sections
were processed through the routine tissue processor. The stained samples were
examined by means of light microscope for histological changes. Histological
examination showed clumping of cell neurons, or reduced pyramidal cells and scanty
neurofibrillary tangle which was an indication of neurodegeneration in the treated
groups and which may suggest or tend to link accumulation of aluminium on brain
cells to incidence or aetiology of Alzheimer’s disease .The haematological results
showed that the differences in parked cell volume, white blood cell and neutrophils
of the control and experimental groups were statistically significant at P<0.05. The
increase in PCV, neutrophil and WBC observed in this study could be as a result of
the destruction of the hippocampal cells due to oral administration of aluminium
chloride. It was however, concluded that the oral administration of aluminum
~ xiv ~
chloride could induce brain damage which may possibly impair memory and learning
as seen in Alzheimer disease.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL IN
PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE
AWARD OF THE DEGREE OF MASTER OF SCIENCE IN
HUMAN ANATOMY
DEPARTMENT OF HUMAN ANATOMY
FACULTY OF MEDICINE
AHMADU BELLO UNIVERSITY
ZARIA
NIGERIA
FEBRUARY, 2008
Keywords
EFFECT,, ORAL ADMINISTRATION,, ALUMINIUM CHLORIDE, HIPPOCAMPUS (BRAIN),, WISTAR RATS.