EVALUATING GENOMIC DEOXYRIBONUCLEIC ACID (gDNA) OF PARENTS AND OFFSPRING TOWARDS DETERMINING RICKETS HEREDITARY LINK IN GONIN GORA, KASO AND JANKASA COMMUNITIES IN KADUNA STATE
EVALUATING GENOMIC DEOXYRIBONUCLEIC ACID (gDNA) OF PARENTS AND OFFSPRING TOWARDS DETERMINING RICKETS HEREDITARY LINK IN GONIN GORA, KASO AND JANKASA COMMUNITIES IN KADUNA STATE
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Date
2011-03
Authors
ALHAJI, Adamu Sallah
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Abstract
Rickets is still a debilitating disease in various communities of Jankasa, Kaso and
Gonin Gora in which about 3-7 percent of the population is affected. Genomic
Deoxyribonucleic acid (gDNA) of parents and offspring were evaluated towards
determining rickets hereditary link in the population. Four families from Jankasa, Kaso
and two families from Gonin Gora composed of father, mother, child without rickets
and child with rickets each were used for the study.
The DNA were extracted using zymo DNA extraction kits, they were quantified and
purified using Biophotometer and gel electrophoresis respectively. Gel was viewed
under ultra violet light and documented. The DNA was then restricted and amplified
using polymerase chain reaction (PCR) technique.
The blood samples of parents and offspring from Kaso, Jankasa and Gonin Gora
showed bands which indicated the presence of DNA. Lower concentrations of gDNA
were observed for the rickets children who could be due to the fact that blood formation
occurs in the long bone and Rickets is the long bone disease. Comparison of mean
concentration (μg/ml) between rickets and non Rickets afflicted children using T-test
gives 1.6 calculated as opposed 1.73 tabulated suggesting no significant difference at
95% confidence interval.
The isolated DNA from all blood samples were restricted, the restricted fragment
length polymorph of DNA used in PCR showed that the vitamin D receptor (VDR)
gene for hereditary vitamin D resistant rickets (HVDRR) was successfully amplified in
rickets children and gave smear amplification in parents and children without rickets at
the same PCR cycle.
The study concluded that there is an hereditary link in the rickets disorder affecting
Kaso, Jankasa and Gonin Gora communities. .
Description
A THESIS SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES
AHMADU BELLO UNIVERSITY IN PARTIAL FULFILLMENT OF THE
REQUIREMENT FOR THE AWARD OF M.Sc. IN PHARMACEUTICAL AND
MEDICINAL CHEMISTRY
DEPARTMENT OF PHARMACEUTICAL AND MEDICINAL CHEMISTRY,
FACULTY OF PHARMACEUTICAL SCIENCES AHMADU BELLO
UNIVERSITY
ZARIA, NIGERIA
Keywords
EVALUATING GENOMIC DEOXYRIBONUCLEIC ACID,, PARENTS,, OFFSPRING,, DETERMINING RICKETS HEREDITARY LINK,, GONIN GORA,, KASO,, JANKASA COMMUNITIES,, KADUNA STATE.