EFFECTS OF DIFFERENT AVIAN EGG YOLKS AND VITAMIN C INCLUSION IN TRIS-SODIUM CIRATE EXTENDER ON CHARACTERISTICS OF CHILLED BOVINE SEMEN
EFFECTS OF DIFFERENT AVIAN EGG YOLKS AND VITAMIN C INCLUSION IN TRIS-SODIUM CIRATE EXTENDER ON CHARACTERISTICS OF CHILLED BOVINE SEMEN
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Date
2014-07
Authors
GOJE, JESSICA BAZACHAT
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Abstract
Two experiments were carried out to evaluate the effect of extenders made from the egg yolk of four avian species (chicken, guinea fowl, turkey and quail) and three inclusion levels of Vitamin C (0, 3 and 6mg/ml) of the best two egg yolk extenders (chicken and quail) on characteristics of bull semen at different storage periods (0, 24, 48 and 72 hours). Both experiments were replicated for five weeks using three Friesian× Bunaji bulls respectively. The result of the first experiment shows that egg yolks had significant effect (P<0.05) on motility, viability and total abnormalities of bovine spermatozoa. Chicken egg yolk Tris-sodium citrate extender had significantly higher (P<0.05) motility andviability with lower (P<0.05) total abnormalities after 72 hours. Turkey egg yolk extender had significantly lower (P<0.05) motile, viable sperm cells and higher (P<0.05) total abnormalities. Quail egg yolk extender had significantly lower (P<0.05) sperm abnormalities than turkey egg yolk extender though there was no significant difference in their motility after 72 hour. The result of the second experiment shows that the inclusion levels of Vitamin C on chicken and quail egg yolk extender had significant effect (P<0.05) on motility, viability, normal cells abnormalities of bull spermatozoa but no significant effect (P>0.05) on semen pH at 0, 24, 48 and 72 hours respectively. After 72 hours there was significant higher (P<0.05) motile, viable and normal sperm cells in the quail and egg yolk extenders with higher inclusion level of Vitamin C compared to their control. In conclusion, this experiment reveals that chicken egg yolk extender did best in cryopreservation of bull semen and is followed by quail egg yolk. Also inclusion of Vitamin C up to 6mg/ml in both chicken and quail egg yolk semen extenders maintained semen quality.
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A THESIS SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER OF SCIENCE DEGREE IN ANIMAL SCIENCE. DEPARTMENT OF ANIMAL SCIENCE, FACULTY OF AGRICULTURE,AHMADU BELLO UNIVERSITY, ZARIANIGERIA
Keywords
EFFECTS,, AVIAN EGG YOLKS,, VITAMIN C,, INCLUSION,, TRIS-SODIUM,, CIRATE EXTENDER,, CHARACTERISTICS,, CHILLED BOVINE SEMEN.