A SURVEY OF HUMAN PARAINFLUENZA VIRUS AMONG CHILDREN IN KADUNA METROPOLIS, NIGERIA

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dc.contributor.authorBONIRE, FOLASHADE SARAH
dc.date.accessioned2016-07-21T08:07:26Z
dc.date.available2016-07-21T08:07:26Z
dc.date.issued2015-03
dc.descriptionA THESIS SUBMITTED TO THE POST-GRADUATE SCHOOL, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA. IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN MICROBIOLOGY. DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA. MARCH,en_US
dc.description.abstractRespiratory infections have a significant impact on health worldwide. The great majority of respiratory infections are of viral origin of which greatly includes Human Parainfluenza viruses. They are rapidly growing viruses that cause significant human and veterinary disease. This study was done to carry out a survey of Human Parainfluenza Virus in children aged 1-12years in Kaduna Metropolis, Nigeria using the Enzyme Linked Immunosorbent Assay Diagnostic kits. The seroprevalence was determined to be 76.6%. Throat swabs were obtained from thirty (30) children with respiratory symptoms such as cold, catarrh, cough and fever. The swabs were cultured aseptically in 10-day old embryonated eggs incubated at 37oC for 24 – 48 hours. The allantoic fluid (which was the viral culture) was harvested and tested for the presence of parainfluenza virus using the haemagglutination assay procedure. Of the thirty samples, ten showed agglutination of various titers; 1:256, 1:128, 1:256, 1:16, 1:16, 1:128, 1:128, 1:32, 1:64, 1:64, thereby indicating the presence of the virus with a prevalence of 33.3%. The antibody level in the highly positive sera samples were tested for by using the Haemaglutination Inhibition Method using the viral antigen isolated with titre 1:256. Risk and demographic factors such as Age of the children (χ2=17.408, p=0.001), parental occupation(χ2=10.116, p=0.039),Parental educational status, Vitamin A deficiency, Frequency of eating, Household size, Duration of breastfeeding (χ2=8.439, p=0.015), environmental smoke, respiratory symptoms (χ2=5.116, p=0.024), Fever, sickle cell and underlying diseases were analysed as well. Age, Parental occupation, Duration of breastfeeding, Presence of respiratory symptoms were significantly associated with the infection. An antibody level of 1:64 ie 64HAIunits/ml of blood was found in only one sample. ELISA in this study was discovered to be a more sensitive diagnostic method than Haemagglutination Inhibition. Observation from the study showed the importance of Human Parainfluenza Virus as an agent of respiratory tract infection in children. As antiviral drugs are not readily available, preventive measures such as eating of balanced diet, proper hygienic practices and parental supervision should be adhered to in the control of the infection.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8114
dc.language.isoenen_US
dc.subjectHUMAN PARAINFLUENZA VIRUSen_US
dc.subjectCHILDREN IN KADUNA METROPOLISen_US
dc.subjectNIGERIAen_US
dc.titleA SURVEY OF HUMAN PARAINFLUENZA VIRUS AMONG CHILDREN IN KADUNA METROPOLIS, NIGERIAen_US
dc.typeThesisen_US
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