EVALUATION OF SOME COAGULATION FACTORS IN COCKERELS EXPERIMENTALLY INFECTED WITH VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS (IBDV37/KADUNA NG/2009)

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dc.contributor.authorOYEDEJI, LUKMAN
dc.contributor.authorMUFTAHU
dc.date.accessioned2015-10-19T09:23:48Z
dc.date.available2015-10-19T09:23:48Z
dc.date.issued2015-03
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A DEGREE OF MASTER OF SCIENCE IN VETERINARY PATHOLOGYen_US
dc.description.abstractYoung chickens in Nigeria are known for their high susceptibility to very virulent infectious bursal disease virus (vvIBDV). Almost all clinical cases of infectious bursal disease (IBD) have been associated with haemorrhage. Coagulopathies and thrombocytopaenia have been associated with haemorrhage. This study aimed at evaluating the coagulation defects in IBD. One hundred Near Black cockerels were purchased at day old. They were acclimatized for the period of 28 days before being randomly divided into two groups of 50 chickens each and kept separately. One group of fifty cockerels were innoculated with vvIBDV strain IBDV37\Kaduna NG\2009 (GenBank accession number JX 424062) intraocularly at the age of 35 days. Four and half milliliter of blood was collected to determine the haemogram and coagulation factors at 6, 12, 24, 36, 48, 60, 72, 84, 96, 120 and 168 hours post inoculation (hpi). Necropsy was carried out during sampling and tissue sections of the bursa of Fabricius, spleen, thymus, liver, kidney, keel and thigh muscles were taken for histopathology. The clinical signs observed beginning at 48 hours pi included depression, anorexia, ruffled feathers, and greenish yellow diarrhea. The thrombocyte count reduced from 7.86 ± 0.79 ×103/μl at 6 hpi to 2.55 ± 0.14 ×103/μl at 84 hpi (p ˂ 0.05) in the IBDV innoculated group. Activated partial thromboplastin time revealed prolonged clotting time between 60 hpi and 120 hpi. The major lesions observed at necropsy include bursal oedema and haemorrhage beginning at 72 hpi and later atrophy, petechial and ecchymotic haemorrhages in thigh and keel muscles and at the mucosal junction between proventriculus and gizzard with higher frequencies being observed in dead birds than sacrificed ones. Histopathological examination of bursal sections affirmed that the predominant lesion was lymphoid cell necrosis. The spleen and thymus were much less severely affected and possess greater regeneration potential. The results showed that vvIBDV of Nigerian field isolate caused severe lymphoid cell necrosis and later bursal atrophy and. Haemorrhage is associated with mortality in IBD. The marked thrombocytopaenia observed which could be the cause of the delayed coagulation time contributes significantly to the haemorrhages.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7011
dc.language.isoenen_US
dc.subjectEVALUATIONen_US
dc.subjectCOAGULATIONen_US
dc.subjectFACTORSen_US
dc.subjectCOCKERELSen_US
dc.subjectEXPERIMENTALLYen_US
dc.subjectINFECTEDen_US
dc.subjectVIRULENTen_US
dc.subjectINFECTIOUSen_US
dc.subjectBURSALen_US
dc.subjectDISEASEen_US
dc.subjectVIRUSen_US
dc.titleEVALUATION OF SOME COAGULATION FACTORS IN COCKERELS EXPERIMENTALLY INFECTED WITH VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS (IBDV37/KADUNA NG/2009)en_US
dc.typeThesisen_US
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