OCCURRENCE OF ANTIBACTERIAL RESISTANT-ESCHERICHIA COLI BEARING QUINOLONE RESISTANCE AND EXTENDED SPECTRUM β-LACTAMASES GENES FROM COMMERCIAL POULTRY IN ZARIA, KADUNA STATE, NIGERIA.
OCCURRENCE OF ANTIBACTERIAL RESISTANT-ESCHERICHIA COLI BEARING QUINOLONE RESISTANCE AND EXTENDED SPECTRUM β-LACTAMASES GENES FROM COMMERCIAL POULTRY IN ZARIA, KADUNA STATE, NIGERIA.
No Thumbnail Available
Date
2016-03
Authors
JIBRIL, JIBRIL YAKUBU
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Fluoroquinolones and Extended Spectrum Beta-Lactam (ESBL) antibiotics represent important
classes of antibiotics in both veterinary and clinical practice. Given their efficacy in treating
infections resistant to other drugs, these drugs have enjoyed widespread and often indiscriminate
use. This study was aimed at determining the occurrence of antibacterial resistant E. coli from
poultry and their potential for disseminating plasmid-borne qnrand ESBL gene variants. Twenty
five (25%) of farms that are registered with the poultry clinic of the Veterinary Teaching
Hospital, Ahmadu Bello University, Zaria within the year 2014 were selected using simple
random sampling technique. 30% E. coliisolation rate was recorded. The highest resistance to
fluoroquinolone was seen with enrofloxacin (49.3%) followed by ofloxacin and ciprofloxacin
with 33.3 and 22.7% respectively, while 58.7% were resistant to nalidixic acid. About 93.3, 80
and 76% were respectively resistant to amoxicillin/clavulanic acid, streptomycin and
sulphamethoxazole/trimethoprim. Resistance to chloramphenicol, tetracycline and gentamicin
also respectively accounted for 68, 65.3 and 52%. Least resistance was however seen with
doxycycline (10.7%), nitrofurantoin (6.7%), ceftriaxone (6.7%), imipenem (2.7%) and cefepime
(1.3%). Multidrug resistance was seen in 92% of the isolates tested,all of which had a MAR
index of ≥0.20. qnrS and bla TEM were found in eighteen (24%) of the E. coli isolates. This
study however, showed no significant association between management type and feed type with
fluoroquinolone and ESBL resistance. This study has demonstrated a high prevalence of
multidrug resistance among E. coli isolates from poultry in Zaria. It further revealed the
existence of qnrS and bla TEM,an ESBL resistance encoding gene. It is therefore recommended
that poultry farmers use antibiotics as therapeutic only when necessary in accordance with good
drug use and management practices under the prescription of a certified veterinarian.
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DEGREE OF MASTER OF SCIENCE IN VETERINARY EPIDEMIOLOGY
DEPARTMENT OF VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
ANTIBACTERIAL,, ESCHERICHIA COLI,, QUINOLONE,, EXTENDED SPECTRUM,, β-LACTAMASES GENES,, POULTRY,, ZARIA,