ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM THE AERIAL PARTS OF Maesobotrya barteri [HUTCH]

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dc.contributor.authorOGWUCHE, CHRISTIANA ENE
dc.date.accessioned2016-07-27T08:18:48Z
dc.date.available2016-07-27T08:18:48Z
dc.date.issued2015-10
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN ORGANIC CHEMISTRY DEPARTMENT OF CHEMISTRY AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractThe petroleum ether, chloroform, ethyl acetate and methanol extracts of the aerial plant parts of Maesobotrya barteri were screened for their antimicrobial activity and their chemical constituents. The preliminary phytochemical screening of the various extracts showed the presence of carbohydrates, saponnins, triterpenes, steroids, tannins, flavonoids, and cardiac glycosides. The result of the antimicrobial screening showed that all the extracts had significant activity against most of the microorganisms tested. The minimum inhibitory concentration (MIC), minimum bactericidal concentration and minimum fungicidal concentration (MBC/MFC) of the extracts showed that almost all the organisms tested were responsive to the extracts at a given concentration. It also showed that, ethyl acetate and chloroform crude extracts exhibited the highest activity against the bacterial Salmonellae typhi and Shigella dysenteriae and Candida krusei and Candida stellatoidea for fungi. The controls used were Sparlfloxacin 2mg/ml for the bacteria and Fluconazole 5mg/ml for the fungi. Chromatographic separation of the ethyl acetate and petroleum ether extracts of the aerial plant parts of Maesobotrya barteri afforded two compounds labelled as 1 and 2. These compounds (1 and 2) were characterised by 1D and 2D NMR spectroscopy and identified as β-amyrin and an isomer of betulinic and oleanolic acid. They were obtained from the ethyl acetate and petroleum ether fractions, a white crystalline and yellowish crystalline compound respectively. They showed significant antimicrobial activities. Compound (1) (β-Amyrin) from the ethyl acetate fraction was found to be active against most of the tested microbes and gave a zone of inhibition range between 26 - 32 mm. The MIC value for the most sensitive organisms were recorded at a concentration of 6.25 μg/ml and the other microbes were sensitive at 12.5 μg/ml with a corresponding MBC/MFC recorded at 25 μg/ml for some of the test microbes and 50 μg/ml for the others. Antimicrobial 10 result for compound (2), betulinic acid and oleanolic acid (as isomer) from petroleum ether fraction showed a zone of inhibition range between 24 - 30 mm. The MIC values for the most sensitive organisms were recorded to at a concentration of 6.25 μg/ml and the other microbes were sensitive at 12.5 μg/ml. The activity recorded by the crude and pure isolates on the organisms are in line with the ethnomedicinal claims of the plant based on their zones of inhibition as compared with those of standard drugsen_US
dc.identifier.urihttp://hdl.handle.net/123456789/8197
dc.language.isoenen_US
dc.subjectISOLATION AND CHARACTERIZATIONen_US
dc.subjectBIOACTIVE COMPOUNDSen_US
dc.subjectAERIAL PARTSen_US
dc.subjectMaesobotrya barteri [HUTCH]en_US
dc.titleISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM THE AERIAL PARTS OF Maesobotrya barteri [HUTCH]en_US
dc.typeThesisen_US
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