ABUNDANCE OF INDOOR RESTING MOSQUITO POPULATIONS AND PLASMODIUM FALCIPARUM INFECTION OF ANOPHELES SPP IN SETTLEMENTS AROUND THE ZARIA DAM, ZARIA, KADUNA STATE

Abstract
The species composition of mosquitoes resting indoors in settlements surrounding the Zaria dam, Zaria, namely: Angwan Malami, Angwan Shika and Zaria water works quarters were investigated. Mosquitoes were caught twice a month for ten months January to October (2013),by application of pyrethrium spray in rooms where tenants had slept the previous night. The total mosquito caught were first identified microscopically,the Anopheles gambiaecomplex were morphologically identified. Using the Polymerase Chain Reaction (PCR) technique, the Anopheles gambiaecomplex were identified molecularly and further assyed by Enzyme Linked Immuno-sorbent Assay (ELISA) to determine the sporozoite infection rate. Microscopically, Culex quinquefasciatus, Culex annulioris, Anopheles gambiae, Anopheles funestus, Anopheles rhodesiensis, Anopheles arabiensis and Aedes aegypti were encountered. Culex quinquefasciatuswasthe most abundant with 254 (50.2%), followed by Anopheles species 134 (26.5%.) and only a single species of Aedes aegypti 1( 0.2%) was collected in the study. Anopheles gambiaes.l samples identified by PCR (Polymerase Chain Reaction) were 104 out of which 66( 63.5%) showed the diagnostic fragment (390bp) characteristic of Anopheles gambiaes.s while 27 (26%) showed the diagnostic fragment (315bp) of Anopheles arabiensis. The other 11( 10.5%) of the sample were not amplified. The infectivity rates were 4.3% (2/46), 4.5%(1/22) and 5.1% (1/19) in Angwan Shika, AngwanMalami and Water Works Quarters respectively. The mean human biting rates were 0.84, 0.79 and 0.73 respectively while the entomological inoculation rate is 104.4.This study show An.gambiae, An.arabiensis,Culex quinquefasciatus and Aedes aegyptia abound in the study area. The infection rate, inoculation rate and human biting rate revealed during the study have incriminate An.gambiae and An.arabiensis as the main vectors offalciparummalaria in the settlements.
Description
A THESIS SUMBITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE (M.SC) DEGREE IN ZOOLOGY DEPARTMENT OF BIOLOGICAL SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA. AUGUST, 2014
Keywords
ABUNDANCE,, INDOOR RESTING,, MOSQUITO POPULATIONS,, PLASMODIUM,, FALCIPARUM INFECTION,, ANOPHELES SPP,, SETTLEMENTS,, ZARIA DAM,,, ZARIA,, KADUNA STATE
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