ISOLATION AND MOLECULAR DETECTION OF ANTIBIOTIC RESISTANT SALMONELLA SPECIES FROM SOME POULTRY FARMS IN SOUTHERN PART OF KATSINA STATE, NIGERIA

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dc.contributor.authorBASHIR, Muideen
dc.date.accessioned2017-12-20T11:50:36Z
dc.date.available2017-12-20T11:50:36Z
dc.date.issued2017-06
dc.descriptionA DISSERTATIONSUBMITTED TO THESCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA,IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF AMASTER OF SCIENCE DEGREE IN MICROBIOLOGY. DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractMeat and poultry products are major vehicles for transmitting Salmonella species to human. The study was aimedat isolating and molecularly determining the antibiotic resistance genes of Salmonella in poultry farms in Southern part of Katsina State Nigeria. A purposive sampling technique was employed in the selection of the study area. Three hundred and twenty samples were collected from broiler birds using simple random sampling consisting each of one hundred and sixty cloacal swabs and faecal droppings from four Local Government Areas in southern part of Katsina state, Nigeria. Samples were pre- enriched in buffered peptone water and selectively enriched in selenite cysteine broth. Isolation and identification was done by inoculating the enriched samples onto xylose lysine deoxycholate agar. Confirmation of the presumptive Salmonella colonies was carried out using different biochemical test and Microgen GNA- A identification system. Antibiotic susceptibility testing was performed using standard guidelines according to CLSI (2012). Polymerase chain reaction was used to detect the presence of antibiotic resistance genes in the Salmonella to tetracycline, beta lactams, sulphonamides, trimethoprim and chloramphenicol.Risks factors related to contamination of the farm and drug resistance toSalmonella in the poultry farmswas determined using a structured questionnaire.SalmonellaArizonae was detected from the study with a prevalence of 0.94% from the 320 samples. Statistically, significant difference (p<0.05) was observed between isolation from the different samples collected and from the different farms. All the isolates were 100% resistant to cefuroxime, amoxicillin- clavulanic acid, ciprofloxacin, chloramphenicol, tetracycline, and sulfomethoxazole-trimethoprim. The SalmonellaArizonae possesses resistance genes to tetracycline (66.7%), beta- lactams(100%), sulphonamides (66.7%),trimethroprim (100%), and chloramphenicol (66.7%). Knowledge of salmonellosis (OR13.00; CI 95% 334.50-505.25), leaving dead chicken in the farm (OR1.44; CI 95% 0.04-56.14), sweeping of feed on the ground in the farm (OR3.86; CI 95% 0.12-126.74), contamination of food during transport and storage (OR5.00; CI 95% 0.07-366.35) were found to be associated withSalmonella contamination in the poultry farms. Also, addition of antibiotics to the feed (OR0.73; CI 95% 0.02-25.09), faeces in water (OR0.20; CI 95% 0.003-14.65), washing of hands after handling the birds (OR0.20; CI 95% 0.003-14.65), were found to be protective against Salmonella contamination. Furthermore, addition of antibiotics to the xix feed daily(OR2.60; CI 95% 0.04-170.39), overuse or misuse of drugs (OR13.60; CI 95% 0.02-26.91), sensitivity testing before drug prescription(OR0.26; CI 95% 0.04-170.39), taking of drugs according to prescription (OR13.60; CI 95% 0.02-26.91), practicing ofself-medication(OR2.33; CI 95% 0.67-76.67) and buying of drugs in local shop(OR13.00; CI 95% 334.50-505.25 ) were found to be associated with drug resistant SalmonellaArizonae.The study reveals the presence of multi- drug resistantSalmonellaArizonae in the poultry farms in southern part of Katsina State.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9824
dc.language.isoenen_US
dc.subjectISOLATION AND MOLECULAR DETECTION ,en_US
dc.subjectANTIBIOTIC RESISTANT SALMONELLA SPECIES,en_US
dc.subjectPOULTRY FARMS,en_US
dc.subjectSOUTHERN PARTen_US
dc.subjectKATSINA STATE, NIGERIAen_US
dc.titleISOLATION AND MOLECULAR DETECTION OF ANTIBIOTIC RESISTANT SALMONELLA SPECIES FROM SOME POULTRY FARMS IN SOUTHERN PART OF KATSINA STATE, NIGERIAen_US
dc.typeThesisen_US
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