THE ROLE OF KUPFFER CELL SURFACE LECTIN IN ERYTHROPHAGOCYTOSIS IN BOVINE Trypanosoma vivax INFECTION
THE ROLE OF KUPFFER CELL SURFACE LECTIN IN ERYTHROPHAGOCYTOSIS IN BOVINE Trypanosoma vivax INFECTION
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Date
1992-08
Authors
IBRAHIM,
Najume Doguwar Giginya
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Abstract
Desialylated erythrocytes from experimental Trypanosoma vivax infected goats
and cattle were interacted with their respective homologous isolated liver
kupffer cells in the presence of bovine calf serum, with and without lactose.
The highest percentage of desialylated erythrocytes bound to Kupffer cells
in conjunction with erythrophagocytosis was between 12 and 19% and 13 and 14%
for the infected goats and cattle respectively. But, summing the latter
percent values with regards to Kupffer cells bound to desialylated
erythrocytes without erythrophagocytosis, and the existence of
erythrophagocytosis without binding of red cells, much higher values of 71% and
62% were obtained in the T. vivax infected goats and cattle, respectively,
without trace of lactose.
Interactions of the desialylated erythrocytes from T. vivax infected goats in
the presence of lactose, or T. vivax infected cattle intravenously infused
with 0.5 g per kg body weight lactose, both in the presence of bovine calf
serum, showed remarkable inhibition of both binding and erythrophagocytosis.
Low values of 2 % and 0-1 % were obtained in goats and cattle, respctively. The
inhibitory effect was accomplished through competitive binding of the
kupffer cells surface lectin by lactose. The results suggest strongly that
the erythrophagocytosis in acute ruminant T.vivax anaemia is partly lectin
based, and the significance of these findings were discussed in relation to
the control of trypanosomiasis.
In a separate experiment, 2 out of 4 T. vivax infected, and 2 out of another
4 T. vivax infected-lactose infused Sokoto Gudali calves, including one out of
4 control calves were sacrificed for pathological examinations. Except for the
4 control, the remaining 8 calves were experimentally infected using 11 x
106 T. vivax trypanosomes, intravenously, as a source of neuraminidase. At the
peak of parasitaemia, lactose was intravenously infused at the rate of 0.5
g kg-11 body weight into 4 of the infected calves, to inhibit the binding of
desialylated erythrocytes to kupffer cells
Description
A Thesis submitted to the Postgraduate SchooL, Ahmadu BeLLo University, in
Partial fulfillment of the requirements for the degree of Doctor of
Philosophy (PhD Veterinary Pathology), Department of Pathology and
Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria
- Nigeria.
August, 1997
Keywords
KUPFFER CELL SURFACE LECTIN,, ERYTHROPHAGOCYTOSIS, BOVINE, Trypanosoma vivax INFECTION.