PREVENTIVE EFFECTS OF DIETARY INCLUSION WITH CRASSOCEPHALUM RUBENS (JUSS EX JACQ.) LEAF ON N-METHYL-N-NITROSOUREA-INDUCED COLORECTAL CARCINOGENESIS IN ALBINO WISTAR RATS

Simple item page
dc.contributor.authorALHASSAN, SOLOMON OGUCHE
dc.date.accessioned2018-09-11T10:22:05Z
dc.date.available2018-09-11T10:22:05Z
dc.date.issued2017-07
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractThe ability to prevent the progression of carcinogenesis has been demonstrated in many plants and natural products. This study set out to evaluate the preventive potential of dietary inclusion with Crassocephalum rubens on colon carcinogenesis initiated via N-methyl-N-nitrosourea induction. Forty-two wistar rats were divided randomly into 6 groups (n=7) with treatment groups receiving 0%, 2.5%, 5% and 10% dietary inclusion + MNU for 12 Weeks. The study included three groups of controls namely, MNU control, Normal control and 10% dietary inclusion control. At the end of 12 weeks, parameters such as malondialdehyde, endogenous antioxidants enzymes, carcinoembryonic antigen, haematological, histological and immunohistochemistry of MutL Homologue-1 were evaluated. A statistically significant increase (p≤ 0.05) in the activities of both superoxide dismutase (SOD) and Catalase (CAT) in the colon, liver and kidney of treated groups when compared with MNU control especially at 5% and 10% dietary inclusion. The reverse was observed for the extent of lipid peroxidation measured by assaying for malondialdehyde in the liver and colon of treated groups when compared with MNU control (0.27±0.01 and 0.38±0.04 nmol/ml/mg protein in the liver and colon respectively). An increase in PCV, RBC, haemoglobin count, WBC, total platelet counts and neutrophils were observed, while a decrease in lymphocyte count was observed in treated groups when compared with MNU controls. A statistically significant (p≤ 0.05) decrease in the level of CEA was observed in treated groups compared with MNU control (125±12.42 pg/ml). Histologic and immunohistochemical evaluation of tissue sections also showed a reduction in tissue damage and mild to no expression of the MLH-1 gene in diet included groups. These results point to the ability of the plant to reduce the effects of chemically induced carcinogenesis and serve as basis for recommending its frequent consumption as part of the diet.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10374
dc.language.isoenen_US
dc.subjectPREVENTIVE EFFECTS,en_US
dc.subjectDIETARY INCLUSION,en_US
dc.subjectCRASSOCEPHALUM RUBENS,en_US
dc.subject(JUSS EX JACQ.),en_US
dc.subjectLEAF,en_US
dc.subjectN-METHYL-N-NITROSOUREA-INDUCED COLORECTAL CARCINOGENESIS,en_US
dc.subjectALBINO WISTAR RATSen_US
dc.titlePREVENTIVE EFFECTS OF DIETARY INCLUSION WITH CRASSOCEPHALUM RUBENS (JUSS EX JACQ.) LEAF ON N-METHYL-N-NITROSOUREA-INDUCED COLORECTAL CARCINOGENESIS IN ALBINO WISTAR RATSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Oguche Final thesis for submission 2nd Augus.pdf
Size:
3.04 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
SCIENCE