EVALUATION OF PHARMACOGNOSTIC PARAMETERS AND HEPATOTOXIC EFFECTS OF EXTRACTSOF CASSYTHA FILIFORMIS LINN ON PARACETAMOL-INDUCED LIVER DISORDERS IN WISTER RATS

Simple item page
dc.contributor.authorADAMU, Aminu Ambi
dc.date.accessioned2014-09-26T07:52:44Z
dc.date.available2014-09-26T07:52:44Z
dc.date.issued2014-01
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES,AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY DEGREE IN PHARMACOGNOSY DEPARTMENT OF PHARMACOGNOSY AND DRUG DEVELOPMENT, FACULTY OF PHARMACEUTICAL SCIENCES AHMADU BELLO UNIVERSITY, ZARIA NIGERIAen_US
dc.description.abstractCassytha filiformis, a leafless and perennial vine with small scales as a replacement of the leavesis currently being used in the treatment of various disease conditions including jaundice without standardization.Microscopical evaluation, chromatographic analysis (TLC, HPLC, LC-MS/MS), spectroscopic analyses (NMR, FTIR, MS) and hepathoprotective studies were carried out with the view to scientifically verify the potentials of this plant used in traditional medicine. The results revealed the presence of some diagnostic microscopical features such as paracytic stomata, unicellular covering trichomes with cystoliths, prismatic calcium oxalate crystals and annular xylem vessels. Quantitative physical constants include moisture contents (5.5 %), ash value (17 %), acid insoluble ash value (1 %), total tannins (27.3 %), swelling index (165 %), water, alcohol and oil extractive indices (20.6 %, 13.6 % and 1.6 % respectively). Trace metals detected in C. filiformis such as Fe (165.4279 ppm), Mn (14.4093 ppm) and Ni (2.7933 ppm) which are essential were higher than FAO/WHO (1984) permissible limit for edible plants. While others:Pb (0.0568 ppm) Zn (0.1094 ppm), Cd (0.0103 ppm) and Cu (0.0535 ppm) were found to be within the safety limit. Preliminary phytochemical screening of the plant signifies the presence of alkaloids, tannins, flavonoids, saponins and steroids Phytochemical constituents identified in ethyl acetate and methanol extracts of C. filiformis include 3,3,O-di-O-methyl ellagic acid, catechin, chalcone compounds, p-hydroxybenzoic acid, isorhamnetin 3-O-rutinoside or isorhamnetin 3-O- neohesperidoside, kaemferol 3 rutinoside and 2-{cyclohex-2-en-1-yl(hydroxyl)methyl}-3-hydroxy-4-(2-hydroxyethyl)-3-methyl- oxoprolinate while that of methanol fraction include 3,3,O-di-O-methyl etllagic acid, methy2-{cyclohex-2-ene-1-y(hydroxyl)methyl}-3-hydroxy-4-(2-hydroxyethyl)-3-methyl-5-oxoprolinate, kaemferol 3 rutinoside, rutin and cathechinas revealed by the library search on LC-MS/MS. Other compounds β-sitosterol and stigmasterol wereisolated from petroleum ether extracts.The petroleum ether extract (500 mgkg-1) and methanol (500 and 1000 mgkg-1) exhibited hepatoprotection properties on wistar albino rats. These results could serve as bases for the use of the plant in traditional medicine for the prevention of liver disorders.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5407
dc.language.isoenen_US
dc.subjectEVALUATION,en_US
dc.subjectPHARMACOGNOSTIC,en_US
dc.subjectPARAMETERS,en_US
dc.subjectHEPATOTOXIC,en_US
dc.subjectEXTRACTSOF CASSYTHA,en_US
dc.subjectFILIFORMIS,en_US
dc.subjectPARACETAMOL-INDUCED LIVER,en_US
dc.subjectDISORDERS IN WISTER RATSen_US
dc.titleEVALUATION OF PHARMACOGNOSTIC PARAMETERS AND HEPATOTOXIC EFFECTS OF EXTRACTSOF CASSYTHA FILIFORMIS LINN ON PARACETAMOL-INDUCED LIVER DISORDERS IN WISTER RATSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
EVALUATION OF PHARMACOGNOSTICPARAMETERS AND HEPATOTOXIC EFFECTS OF EXTRACTSOF CASSYTHA FILIFORMIS LINN ON PARACETAMOL-INDUCED LIVER DISORDERS IN WISTER RATS.pdf
Size:
3.57 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.58 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
PHARMACEUTICAL SCIENCES