PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF THE ROOT PART OF PACHYSTELA BREVIPES (BAKER)BAILL ENGL (SAPOTACEAE
PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF THE ROOT PART OF PACHYSTELA BREVIPES (BAKER)BAILL ENGL (SAPOTACEAE
No Thumbnail Available
Date
2015-05
Authors
EZURUIKE, IKEAGWUGHICHI TIMOTHY
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
ABSTRACT
The root of Pachystela brevipes, which has some traditional medicinal applications was extracted and investigated using Petroleum ether, Chloroform, Ethyl acetate and Methanol. The results of the phytochemical analysis showed the presence of carbohydrates, cardiac glycosides, saponins, steroids/ triterpenes, flavonoids, tannins and alkaloids. The crude extracts showed zones of inhibition in the range, 16-19 mm for Petroleum ether fraction, 20-24 mm for Chloroform, 20-27 mm for Ethylacetate and 20-22 mm for Methanol, against thirteen test organisms; Methicillin Resistant Staphylococcus aureus, Staphylococcus aureus, Escherichia coli; Salmonella typhi, Shigella dysenterea, Pseudomonas aeruginosa, Klebsiella pneumonia, Candida stellatoidea, Candida tropicalis, Candida krusei, Proteus mirabilis, Proteus vulgaris and Streptococcus feacalis. The minimum inhibitory concentration (MIC) of 2.5 mg/mL was recorded for the chloroform, ethylacetate and methanol fraction against the entire test organism except four;S. pyogenes, C. krusei, P. mirabilis and P. vulgaris. The Petroleum ether fraction had Minimum Inhibitory Concentration (MIC) at a concentration of 5.0 mg/mL on eleven test organisms except S. pyogenes, C. krusei, P. mirabilis and P. vulgaris.The minimum bactericidal/fungicidal concentration (MBC/MFC) determination showed that concentrations of 5-10 mg/mL of the petroleum ether, chlororform, ethylacetate and methanol fractions could completely kill the test organismsused in this work except S. pyogenes, C. krusei, P. mirabilis and P. vulgaris. Zymosterol (3β-hydroxy-4β-methyl-5α-cholesta-8, 24-diene-4α-carboxylic acid) was isolated and purified from the ethyl acetate fraction (EAF) and confirmed purely by spectral techniques (1H NMR, 13C NMR and 2D NMR) as well as by comparison with the reported authentic data. This is the first time of isolating Zymosterol from the Sapotaceae family.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR THE DEGREE OF MASTER OF SCIENCE (M. Sc.) IN ORGANIC CHEMISTRY.
DEPARTMENT OF CHEMISTRY,
FACULTY OF SCIENCE,
AHMADU BELLO UNIVERSITY,ZARIA.
Keywords
PHYTOCHEMICAL, ANTIMICROBIAL SCREENING, ROOT PART, PACHYSTELA BREVIPES, BAILL ENGL