DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF RISPERIDONE IN PURE AND TABLET DOSAGE FORMS
DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF RISPERIDONE IN PURE AND TABLET DOSAGE FORMS
No Thumbnail Available
Date
2015-08
Authors
OLOYEDE, Rukayyat Bukola
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Two simple, sensitive, accurate and extraction-free spectrophotometric methods were
developed and described for the determination of risperidone in pure and in tablet dosage
forms. The methods are based on the formation of ion-pair complex between risperidone
and the dyes bromocresol green in method A and thymol blue in method B at room
temperature to form yellow coloured products having absorption maxima at 414 nm and
404 nm respectively. The composition of the ion-pairs was established by Job‟s method
and it was found to be 1:1 for both methods. Different variables affecting the reaction
conditions such as diluting solvents, concentration of dye, reaction time were studied and
optimized. Under the optimal conditions, linear relationship with good correlation
coefficients (0.994 and 0.995 for methods A and B respectively) was found between
absorbance and the concentrations of risperidone in the range of 2-20 µg/ml and 20-40
µg/ml respectively. The assay limits of detection (LOD) and limits of quantification (LOQ)
were 1.27 and 3.84 µg/ml for method A and 7.00 and 21.15 µg/ml for method B. The
precision of both methods did not exceed 15% likewise the percentage relative error was
within the accepted range of 1-5%. No interference could be observed from the excipients
commonly present in tablet or liquid dosage forms. The methods developed have been
validated and there is no significant difference (P 0.05) between the methods and the
reference (BP) method. The methods can be successfully applied for the analysis of
risperidone in pure and tablet dosage forms.
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTERS DEGREE IN PHARMACEUTICAL AND MEDICINAL CHEMISTRY
DEPARTMENT OF PHARMACEUTICAL AND MEDICINAL CHEMISTRY FACULTY OF PHARMACEUTICAL SCIENCES AHMADU BELLO UNIVERSITY, ZARIA NIGERIA
Keywords
SPECTROPHOTOMETRIC, RISPERIDONE, TABLET DOSAGE FORMS