THE HISTOLOGIC EFFECT OF FIXATIVES ON THE STAINING PROPERTIES OF Hibiscus sabdariffa, Sorghum bicolor and Garcinia livingstonei.
THE HISTOLOGIC EFFECT OF FIXATIVES ON THE STAINING PROPERTIES OF Hibiscus sabdariffa, Sorghum bicolor and Garcinia livingstonei.
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Date
2010-10
Authors
OMOKARO, Kenneth Nosa Osagie
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Abstract
This study describes the effect of the fixatives: Buffered neutral formalin, Heidenhains SUSA fixative, Calcium acetate formalin, Muller’s fixative, Helly’s fixative and Carnoy’s fixative on the tissue staining ability of the extracts of Hibiscus sabdariffa, Sorghum bicolor and Garcinia livingstonei. Tissues were fixed in the fixatives, processed by paraffin wax method and the sections were stained with alcoholic extract of Hibiscus sabdariffa and aqueous extracts of Sorghum bicolor and Garcinia livingstonei. Hibiscus sabdariffa gave a good tissue staining contrast and cellular clarity on both the nucleus and cytoplasm. Sorghum bicolor and Garcinia livingstonei also gave a good tissue staining contrast particularly for the cytoplasm, hence they are cytoplasmic stains and may be used as substitutes for eosin in routine histological preparations. The effect of the fixatives varied the tissue staining ability of the local stains, with Carnoy’s fixative emerging as the fixative of choice in this study.
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A THESIS SUBMITTED TO THE POST-GRADUATE SCHOOL
AHMADU BELLO UNIVERSITY
IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN VETERINARY ANATOMY
DEPARTMENT OF VETERINARY ANATOMY
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA
Keywords
EFFECT OF FIXATIVES,, STAINING PROPERTIES,, Hibiscus sabdariffa,, Sorghum bicolor,, Garcinia livingstonei.