EFFECT OF OMEGA 3 FATTY ACIDS AND VITAMIN A ON CARRAGEENAN INDUCED JOINT INFLAMMATION IN FEMALE WISTAR RATS
EFFECT OF OMEGA 3 FATTY ACIDS AND VITAMIN A ON CARRAGEENAN INDUCED JOINT INFLAMMATION IN FEMALE WISTAR RATS
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Date
2018-09
Authors
IDRIS, Rahmatu Ibrahim
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Abstract
Joint inflammation is a hallmark of diseases such as arthritis affecting single or multiple joints associated with increased pro-inflammatory markers and free radicals. Efforts are channeled towards discovering therapeutic agents which are more effective, less toxic with prolonged use and more affordable.The aim of this study was to investigate the effect of omega 3 fatty acids and vitamin A supplements on knee circumference, TNF-α, C-reactive protein (CRP), oxidative stress biomarkers: superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) and malondialdehyde (MDA) on carrageenan-induced joint inflammation in female Wistar rats.Twenty four female Wistar rats of age 10 weeks weighing between 150-200g divided into six groups of 4 animals each were used for the study.Group 1 (normal control), group 2 (injection control [normal saline]), group 3-6 received 0.02ml of 1% CGN injection 3x/week for 10 weeks but in groups 4-6, after first 2 weeks of CGN injection, oral treatment with 200mg/kg Omg3, 4000IU/kg VA and 200mg/kg Omg3+4000IU/kg VA respectively was given daily with simultaneous injection of the CGN for the remaining 8 weeks. Knee diameter was measured before the first injection (at day 0), at week 2 and thereafter weekly for 8 weeks using veneercalliper. Animals were euthanized using ketamine injection and blood samples of 5 ml were drawn from the heart by cardiac puncture, collectedinplane tubes, allowed to clot and serum was separated by centrifugation. Serum TNF-α, CRP, SOD, GSH, CAT and MDA were determined. There was a significant interaction in knee circumference between time and treatment (P<0.05). TNF-α showed a significant decrease (P<0.05) in groups 4, 5 and 6 when compared to group 3. CRP level was significantly decreased (P<0.05) in group 4 and 6 when compared to group 3. There was a significant increase (P<0.05) in Groups 4, 5 and 6 of SOD, GSH and CATwhen compared to group 3. There was a significant decrease (P<0.05) in MDA in
groups 4, 5 and 6 when compared to group 3. In conclusion treatment with combination of 200mg/kg omega 3 fatty acidsand 4000IU/kg vitamin A was more effective than vitamin A lone. Combination of omega 3fatty acids and vitamin A did not show a better outcome than Omega 3 fatty acidalone.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN HUMAN PHYSIOLOGY
DEPARTMENT OF HUMAN PHYSIOLOGY,
FACULTY OF BASIC MEDICAL SCIENCES,
COLLEGE OF HEALTH SCIENCES,
AHMADU BELLO UNIVERSITY,
ZARIA
Keywords
EFFECT,, OMEGA 3 FATTY ACIDS,, VITAMIN,, CARRAGEENAN INDUCED,, JOINT INFLAMMATION,, FEMALE WISTAR RATS