ISOLATION OF CHEMICAL CONSTITUENTS, ANTIBACTERIAL STUDIES OF THE LEAVES OF Globimetulaoreophila (Oliv.) van Tiegh AND POTENTIAL COMBINATION EFFECT WITH CIPROFLOXACIN
ISOLATION OF CHEMICAL CONSTITUENTS, ANTIBACTERIAL STUDIES OF THE LEAVES OF Globimetulaoreophila (Oliv.) van Tiegh AND POTENTIAL COMBINATION EFFECT WITH CIPROFLOXACIN
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Date
2017-01
Authors
SADA, Hadiza
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Abstract
Globimetula oreophila(Loranthaceae) growing on Citrus aurantifolia is a mistletoe used widely in various cultures for the treatment of cancer, hypertension, diabetes, arthritis, heart problems, insomnia, infertility and pneumonia.Shade-dried powdered plant materialwas extracted using three methods; cold maceration, Microwave assisted extraction (MAE) and Polarity based extraction (PBE). The extractions afforded nine extracts: Crude ethanol (CEE=21.40g) from maceration; hexane (HEX=5.0g), Chloroform (CHL= 5.6 g), ethyl acetate (EA=6.9g) and ethanol (EE=26g) from MAE; and ethyl acetate (EA2=7.0g), chloroform (CHL2=7.5g), chloroform-ethanol (CE=1.14g) and ethanol (EE2=15.6g) from PBE..Repeated gel filtration of extract CE on sephadex LH-20 column eluting with methanol resulted in the isolation of two flavonoids;7-methoxyquercetin and Quercetin. The structures of these compounds were elucidated on the basis ofspectroscopic techniques (UV, FTIR, 1D and 2D NMR) and comparison with literature data. Antimicrobial Studies was done using the agar well diffusion method which shows that CEE and EE1 had strong antimicrobial activity against all the tested organisms with zones of inhibition ranging from 10-27mmbut has no activity againstE. coli.All the extracts showed strong activity against K. pneumoniae except extract HC which has no activity against all the isolates. The isolated compounds showed activity against all the bacterial strains except E. coli and VRSA.The extracts and isolated compounds showed potent synergistic activity with ciprofloxacin against the tested organisms.The findings of the study suggests that all the extracts tested have strong antimicrobial activity which might be due to the isolated phytochemical constituents.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTER OF SCIENCE DEGREE IN ORGANIC CHEMISTRY DEPARTMENT OF CHEMISTRY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA-NIGERIA
Keywords
CHEMICAL CONSTITUENTS,, ANTIBACTERIAL STUDIES,, Globimetulaoreophila (Oliv.),, CIPROFLOXACIN,, LEAVES,, van Tiegh,