PRODUCTION OF ALPHA AMYLASE BY BACILLUS SUBTILIS USING MAIZE HUSK AS SUBSTRATE

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dc.contributor.authorMADIKA, ABUBAKAR
dc.date.accessioned2018-09-10T09:05:59Z
dc.date.available2018-09-10T09:05:59Z
dc.date.issued2017-08
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUREMENTS FOR THE AWARD OF MASTER DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractBacillus subtilis was isolated and characterized from soil samples of different locations namely flower bed, botanical garden and refuse dump site using conventional cultural and biochemical methods followed by microgen identification. B. subtilis isolates were screened for amylase production using starch agar plate method. Studies were conducted to produce α-amylase by the Bacillus subtilis using maize husk as substrate. Proximate composition of the maize husk substrate was determined following standard procedures described by Association of Official Analytical Chemists. Effects of incubation temperature, initial pH, incubation period and moisture content on the production of α-amylase were investigated. Alpha amylase was producedby the best α-amylase producing B. subtlis isolate using maize husk as substrate by solid state fermentation under predetermined optimum fermentation conditions. Nine (9) isolates were confirmed to be B. subtilis with percentage occurrence of 80% each from refuse dump site and botanical garden with only 20% from flower bed. All the B. subtilis isolates demonstrated amylase production ability while isolate RD4 from refuse dump site had the highest diameter of zone of starch hydrolysis (29mm). The proximate composition of the maize husk revealed percentages of ash (4.83%), crude protein (7.00%) and carbohydrates (60.37%) contents. All parameters optimized were found to have significantly (p< 0.05) influenced α-amylase production using maize husk. An overall yield of 51.65±0.13 U/g of α-amylase was obtained under predetermined optimum fermentation conditions (35oC, pH 7.0, 72hours and moisture content of 1:2). Bacillus subtilis can readily be isolated from various soil types with frequency of 80% in soils from garden and refuse dumps. Maize husk contains sufficient organic nutrients for growth and amylase production by B. subtilis. The study reveals the potential of maize husk as substrate in the production of alpha amylase by B. subtilis.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10336
dc.language.isoenen_US
dc.subjectPRODUCTION,en_US
dc.subjectALPHA AMYLASE,en_US
dc.subjectBACILLUS SUBTILIS USING MAIZE HUSK,en_US
dc.subjectSUBSTRATEen_US
dc.titlePRODUCTION OF ALPHA AMYLASE BY BACILLUS SUBTILIS USING MAIZE HUSK AS SUBSTRATEen_US
dc.typeThesisen_US
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