SEROPREVALENCE AND MOLECULAR IDENTIFICATION OF MALARIA PARASITES AMONG BLOOD DONORS IN KADUNA STATE, NIGERIA

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dc.contributor.authorDZINGINA, Garba Douglas
dc.date.accessioned2017-10-16T08:00:52Z
dc.date.available2017-10-16T08:00:52Z
dc.date.issued2016-11
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.en_US
dc.description.abstractThis research was undertaken to determine the seroprevalence and molecular identification of malaria parasites among blood donors in Kaduna State, Nigeria. Blood specimens were obtained from 360 blood donors for detection and identification using Giemsa stained thick and thin blood films, rapid diagnostic test kit and Polymerase chain reaction. A total of 27 (7.5%) of the subjects had malaria parasitaemia detected by microscopy with a mean parasite density of 126/μl of blood. The rapid diagnostic test (RDT) kit detected 31 (8.6%) positive cases. Plasmodium falciparum was determined to be responsible for all the cases. There were no mixed infections and no other blood parasites were observed. Malaria parasitaemia in blood donors was not significantly associated with the risk factors considered except occupation and blood group. Also, malaria parasitaemia had no significant effect on the haematological parameters considered. Compared with microscopy, the RDT kit was 70% sensitive and 97% specific. Out of the 23 cases tested by the polymerase chain reaction (PCR), six (26.1%) were positive. The species of the parasite was further confirmed through sequencing of the complete mitochondrial (mt) genome to be Plasmodium falciparum with 97% identity and accession number KT119883.1These findings indicate the need for blood banks to include malaria parasite species screening as a routine test of blood from donors to avert its deadly complications.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9273
dc.language.isoenen_US
dc.subjectSEROPREVALENCE,en_US
dc.subjectMOLECULAR IDENTIFICATION,en_US
dc.subjectMALARIA PARASITES,en_US
dc.subjectBLOOD DONORS,en_US
dc.subjectKADUNA STATE,en_US
dc.subjectNIGERIA
dc.titleSEROPREVALENCE AND MOLECULAR IDENTIFICATION OF MALARIA PARASITES AMONG BLOOD DONORS IN KADUNA STATE, NIGERIAen_US
dc.typeThesisen_US
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