DETECTION AND SURVEY OF BLACKEYE COWPEA MOSAIC VIRUS IN ZARIA AND ENVIRONS
DETECTION AND SURVEY OF BLACKEYE COWPEA MOSAIC VIRUS IN ZARIA AND ENVIRONS
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Date
1996-11
Authors
KASHINA, BONIFACE DAVID
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Abstract
A two-year survey of the incidence of blackeye cowpea mosaic
virus (B1CMV) in Zaria and its environs revealed that the virus is
widespread. Incidence levels and distribution of serotypes varied
from location to location and year to year.
When forty cowpea lines were screened for resistance against
the virus, results obtained from glasshouse screening were more
consistent than those obtained from field screening. Twenty-one
lines tested positive to both serotypes of the virus in the
glasshouse while twenty and twenty one lines respectively tested
positive during the field screening in 1993 and 1994 respectively.
Assessment of the reaction of the forty cowpea lines to BICMV
infection indicated that twenty were resistant, eighteen were
moderately susceptible and only two were highly susceptible.
Significant yield loss was recorded only in three cowpea lines: IT
860-715, TVU 1582 and KVX 402-5-2.
Infectivity of the virus was lost at temperatures above 60cc;
at a dilution of above 10 and after storage of virus sap for more
than 54 hours at room temperature (25°c).
The virus was transmitted by Aphis craccivora Koch, in a nonpersistent
manner. It was acquired after 15 seconds of feeding on
virus source. The virus was also transmitted after 15 second of
feeding on susceptible cowpea plants. Similarly, preliminary
fasting for 15 to 30 minutes was efficient in increasing the
efficiency of transmission.
It is concluded that BICMV is widespread and therefore
requires more study for incorporating its resistance into cowpea
Description
A thesis submitted to the Postgraduate School,
Ahmadu Bello University, Zaria in partial
fulfillment of the requirements for the degree of
Master of Science in crop protection
DEPARTMENT OF CROP PROTECTION
FACULTY OF AGRICULTURE
AHMADU BELLO UNIVERSITY
ZARIA, NIGERIA
NOVEMBER, 1996
Keywords
DETECTION,, SURVEY,, BLACKEYE,, COWPEA,, MOSAIC,, VIRUS,, ZARIA,, ENVIRONS.