EVALUATION OF THE ANTIDIABETIC EFFECT OF BASELLA ALBA LEAF SUPPLEMENT ON STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS
EVALUATION OF THE ANTIDIABETIC EFFECT OF BASELLA ALBA LEAF SUPPLEMENT ON STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS
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Date
2015-12
Authors
HARUNA, Israel Ocheje
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Abstract
The present study evaluated the antidiabetic effect of Basella alba leaf food supplement on streptozotocin-induced diabetic Wistar rats. Twenty five mixed sex Wistar rats weighing between 150-170g were grouped into five groups of five animals each namely; Group A (normal control) non–diabetic rats were fed with normal rat feed; Group B (diabetic control) diabetic rats were fed with normal rat feed; Group C (standard control) diabetic rats received 1mg/kg body weight of glibenclamide orally daily; Group D (treated group) diabetic rats were fed with 10% (w/w) of Basella alba leaf supplement; Group E (treated group) were fed with 20% (w/w) of Basella alba leaf supplement. At the end of 21 days feeding experiment there was a significant (p<0.05) higher blood glucose level in diabetic untreated group when compared with normal control and treated groups. Groups fed with Basella alba leaf had a significant (p<0.05) lowered blood glucose level. There was a significant (p<0.05) low serum insulin level in diabetic untreated group when compared with normal control and treated groups. Also, there was a significant (p<0.05) decrease in serum insulin level in the Basella alba leaf supplemented feed when compared to the normal control group. Among the Basella alba supplemented treated group there was no significant (p>0.05) difference. Histopathology of the pancreas of the diabetic group showed necrotic islets of the cells compared to the normal control. Group treated with 20% Basella alba leaf showed moderate necrosis, all other treated group showed no difference when compared with diabetic group. Histopathology of the liver of the diabetic group shows necrosis of hepatocytes and kupfer cell hyperplasia compared to the normal control. We concluded from the study that Basella alba leaves have hypoglycemic effect on diabetic rats, although the plant leaf did not reverse the damage effect on the histopathology of the liver and pancreas within the time of study.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD
OF A
MASTER OF SCIENCE IN BIOCHEMISTRY
DEPARTMENT OF BIOCHEMISTRY,
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA
Keywords
EVALUATION,, ANTIDIABETIC EFFECT,, BASELLA ALBA,, LEAF SUPPLEMENT,, STREPTOZOTOCIN-INDUCED DIABETIC,, WISTAR RATS,