LARVICIDAL POTENTIAL OF EXTRACTS OF Persea americana SEED AND Chromolaena odorata LEAF AGAINST Aedes vittatus MOSQUITO
LARVICIDAL POTENTIAL OF EXTRACTS OF Persea americana SEED AND Chromolaena odorata LEAF AGAINST Aedes vittatus MOSQUITO
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Date
2015-11
Authors
UMAR, Suleiman Albaba
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Abstract
The larvicidal activity of various solvent (ethanol, ethyl acetate and n-hexane) extracts of Persea americana seed and Chromolaena odorata leaves against Aedes vittatus mosquito was analysed. The most potent solvent (n-hexane) extracts of both plants were fractionated using column chromatography and most effective fractions isolated and identified using Gas Chromatography Mass Spectrometry and Fourier Transform Infrared techniques. Phytochemical screening revealed the presence of steroids, cardiac glycosides and terpenoids in all the extracts. The larvicidal bioassay of Persea americana seed gave LC50 values of 0.827ppm, 1.799ppm and 2.732ppm for n-hexane, ethanol and ethyl acetate extracts respectively, while, Chromolaena odorata leaf extract had LC50 values of 1.835ppm, 3.314ppm, and 5.163ppm for n-hexane, ethanol and ethyl acetate respectively. Column chromatographic fractionation of most potent n-hexane (crude) extracts of both plants, showed increased activity in some of the fractions of Persea americana (nHPa6) and Chromolaena odorata (nHCo6) which showed higher mortality, with LC50 values of 0.486ppm and 1.308ppm respectively. GC/MS analysis of components in nHPa6 and nHCo6 showed oleic acid as the most abundant, in fractions of both plants. The FTIR analyses of nHPa6 and nHCo6 showed absorption bands of the functional groups present, which included; alcohol, alkane, alkene, alkyl halide, aldehyde, carboxylic acid and carbonyl ester, thus, supporting the GCMS result. The n-hexane, ethanol and ethyl acetate extracts of P. americana seed and C. odorata leaves have shown good larvicidal activity and should therefore be further exploited for the control of mosquito larvae.
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A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT FOR THE AWARD OF MASTER OF SCIENCE DEGREE (M.Sc.) IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY, FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.
Keywords
LARVICIDAL POTENTIAL,, EXTRACTS,, Persea americana SEED,, Chromolaena odorata LEAF,, Aedes vittatus MOSQUITO,