EVALUATION OF SERUM TOTAL ANTIOXIDANT STATUS IN TYPE II DIABETIC NIGERIANS
EVALUATION OF SERUM TOTAL ANTIOXIDANT STATUS IN TYPE II DIABETIC NIGERIANS
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Date
2011-07
Authors
YAKUBU, SARAH
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Abstract
Patients with type II diabetes mellitus are more prone to diabetic complications and oxygen
free radicals are known to contribute to the development of complications but there are
conflicting reports regarding antioxidant status in type II diabetic patients. The current
cross sectional study was designed to evaluate the serum total antioxidant status (TAS) in
type II diabetic patients and age matched control subjects in ABUTH Shika, Zaria. Also to
assess their correlation with clinical parameters and biochemical analytes. A total of 281
subjects were recruited for the study. These comprised of 181 type II diabetic patients and
100 controls. Fasting blood glucose (FBG) was measured using glucose oxidase method,
glycated haemoglobin (GHbA1c) using micro column method, TAS using Mosmann method
while vitamins C and E using Moeslinger and Dahot methods respectively. The reference
values of TAS, vitamins C and E are 15.7 – 63.1 mmol/L, 0.2 – 7.0 mg/ml and 3.7- 119.3
g/L respectively. The mean value of TAS (33.3 ± 0.5 mmol/L) was significantly lower (p <
0.05) in diabetic patients than in control subjects (39.4 ± 0.8 mmol/L). While on the other
hand the mean values of FBG and GHbA1c in diabetic patients (7.0 ± 0.3 and 8.2 ± 0.2)
were significantly higher (p< 0.05) than corresponding values in controls (4.1 ± 0.8 and 5.0
±0.1) respectively. The mean values of TAS were however similar (p > 0.05) among
diabetic patients with good and poor glucose control (33.3 ± 0.7 mmol/L versus 32.2 ± 0.7
mmol/L). Also the mean values of TAS were similar (p > 0.05) among diabetic patients
with good and poor GHbA1c control ( 33.4 ± 0.6 mmol/L versus 33.1 ± 0.6 mmol/L).
Similarly, the mean values of TAS in diabetic patients with complications and those without
complications (32.8 ± 0.6 mmol/Lversus 34.4 ± 1.1 mmol/L) were not significantly
different(p > 0.05). The mean value of vitamin E was found to be significantly higher (p <
0.05) in patients with good glucose control (44.6 ±3.0 g/L) and glycated haemoglobin
control (44.5 ± 2.7 g/L) than in those with poor glucose control (41.8 ±2.7 g/L) and glycated
haemoglobin control (41.7± 2.8 g/L). Furthermore, the mean level of vitamin E in patients
without complications (49.5 ±7.0 g/L) was significantly higher (p < 0.05) than in those with
complications (42.5±2.1 g/L). In diabetic patients, there were positive and significant
correlations between TAS and age (r=0.173, p<0.05) as well as duration of diabetes mellitus
(r=0.240, p<0.05). These results suggest that type II diabetic patients of the study area have
low serum level of TAS.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL
FULLFILMENT OF THE REQUIREMENTS FOR THE
AWARD OF MASTERS DEGREE
IN CHEMICAL PATHOLOGY.
Department of Chemical Pathology,
Faculty of Medicine,
Ahmadu Bello University,
Zaria, Nigeria
Keywords
EVALUATION OF SERUM,, SERUM TOTAL ANTIOXIDANT,, TYPE II DIABETIC NIGERIANS