ISOLATION, ANATOMICAL DISTRIBUTION AND ANTIFUNGAL SUSCEPTIBILITY OF DERMATOPHYTES AFFECTING HORSES IN KWARA STATE, NIGERIA

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dc.contributor.authorBALOGUN, RASHIDAT BOLANLE
dc.date.accessioned2015-11-10T09:22:58Z
dc.date.available2015-11-10T09:22:58Z
dc.date.issued2015-06
dc.descriptionISOLATION, ANATOMICAL DISTRIBUTION AND ANTIFUNGAL SUSCEPTIBILITY OF DERMATOPHYTES AFFECTING HORSES IN KWARA STATE, NIGERIA By RashidatBolanle BALOGUN, DVM 2010 (ABU) MSc/Vet Med/5162/2011-2012 A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN VETERINARY MICROBIOLOGY DEPARTMENT OF VETERINARY MICROBIOLOGY AHMADU BELLO UNIVERSITY, ZARIA NIGERIA JUNE, 2015en_US
dc.description.abstractDermatophytes are fungi that colonize keratinized tissues of humans and animals. This study was conducted to isolate dermatophytes from both clinical and asymptomatic cases of dermatophytosis in horses in seven Local Government Areas (LGA) of Kwara State and to determine the susceptibility pattern of the dermatophytes isolated from different anatomical sites on horses to five antifungal agents. Ninety-one samples of plucked hair or scales and scrapings from the skin of horses were initially examined directly using microscopy prior to culture for isolation and identification usingSabouraud‟s dextrose agar (SDA), with 5% NaCl and potato dextrose agar as media for dermatophyte culture and isolation, respectively. Identification of each isolate was through observation of colonial morphology and microscopic appearance of lactophenol cotton blue stained fungal specimen obtained from culture. The assessment of antifungal susceptibility patterns of the dermatophytes isolated was by broth microdilution assay using ketoconazole, fluconazole, amphotericin B, griseofulvin and terbinafine as antifungal agents. From all the samples obtained (91 samples), 14(15.4%) were dermatophytes out of which one was from an asymptomatic horse. These dermatophytes were identified as members of Trichophyton(T.)andMicrosporum(M.)genera. The dermatophyte species isolated were T. tonsurans(7.14%) and T. soudanense (7.14%) which are anthropophylicdermatophytes, T. verrucosum(35.7%), M. gypseum (7.14%), M. persicolor (14.2%), M. equinum (7.14%) and M. fulvum (21.4%). Twelve of the 14 dermatophytes were isolated from 85 male horses while the remaining two were from six female horses. Based on anatomical sites, the highest isolation rate was from the limbs (18.7%) and the lowest from the abdomen (10%). However, the differences between the dermatophytes isolated from male and female horses or the different anatomical sites were not statistically significant (p ˃ 0.05). With regards to samples from the seven LGAs,samples collected from Ilorin-East LGAyielded the highest isolation rate (25%) whilst those fromBarutenLGA had the lowest isolation rate (9.1%). The antifungal susceptibility test showed that terbinafine was the most potent drug with the lowest range of MIC values (0.2-6.5μg/ml) followed by amphotericin B which had MIC range of 0.6-4.0μg/ml and then ketoconazole (0.3-9μg/ml), whereas griseofulvin and fluconazole showed the highest MIC ranges of 1.5-8.0μg/ml and 0.6-19.2μg/ml, respectively,indicating that terbinafine was the most efficacious of the five antifungal agents used in this study. Culture and sensitivity tests should be carried out for effective diagnosis and treatment of fungal infections especially in horses.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7124
dc.language.isoenen_US
dc.subjectISOLATIONen_US
dc.subjectANTIFUNGAL SUSCEPTIBILITYen_US
dc.subjectDERMATOPHYTESen_US
dc.subjectAFFECTING HORSESen_US
dc.titleISOLATION, ANATOMICAL DISTRIBUTION AND ANTIFUNGAL SUSCEPTIBILITY OF DERMATOPHYTES AFFECTING HORSES IN KWARA STATE, NIGERIAen_US
dc.typeThesisen_US
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