ISOLATION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM STAGNANT WATER AND ITS SUSCEPTIBILITY TO METHANOLIC EXTRACTS OF CARROT (Daucus carota L.) ROOT AND LEAF IN VITRO BY

Simple item page
dc.contributor.authorYAKUBU, AMINA
dc.date.accessioned2017-01-13T14:06:54Z
dc.date.available2017-01-13T14:06:54Z
dc.date.issued2015-11
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN EDUCATIONAL BIOLOGY DEPARTMENT OF BIOLOGICAL SCIENCES, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.en_US
dc.description.abstractThe present study describes the susceptibility of stagnant water isolates of Staphylococcus aureus in terms of antimicrobial activity. Staphylococcus aureus was isolated from stagnant water bodies located at four different sites i.e. Anguwan Alkali, Kofan Gayan, Kwarbai, and Magajiya areas of Zaria city and the characterization was done using conventional method and Microgen® identification kit. The study demonstrated that S. aureus is present along with S. hominis and S. heamolyticus in stagnant Waters of Zaria city, Nigeria. Fresh roots and leaves of carrot were obtained from local vegetable farmers in markets of Zaria city. The phytochemical screening shows the presence of some phytoconstituents in both the root as well as the leaf extract. The antibacterial potency was initially determined by agar well diffusion method followed by qualitative evaluation of antibacterial activity by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods. The result showed that methanolic extracts of carrot root was more effective than the methanolic extract of carrot leaf which shows no activity. Methanolic extract of carrot root isolates had the highest antibacterial activity with mean inhibition zone diameter ranging from 6.00 ± 8.49 to 31.00 ± 1.41 mm lower than the control antibiotic, Ciprofloxacin (10μg) with the highest mean inhibition zone diameter of 40.00 ± 0.00mm along the columns and rows. This study has demonstrated the effectiveness of carrot root antibacterial activity in inhibiting S. aureus pathogenic bacteria from stagnant water.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8451
dc.subjectISOLATION AND CHARACTERIZATIONen_US
dc.subjectSTAGNANT WATERen_US
dc.subjectSTAPHYLOCOCCUS AUREUSen_US
dc.subjectSUSCEPTIBILITY TO METHANOLIC EXTRACTS OF CARROTen_US
dc.subjectROOT AND LEAF IN VITROen_US
dc.titleISOLATION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM STAGNANT WATER AND ITS SUSCEPTIBILITY TO METHANOLIC EXTRACTS OF CARROT (Daucus carota L.) ROOT AND LEAF IN VITRO BYen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ISOLATION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM STAGNANT WATER AND ITS SUSCEPTIBILITY TO METHANOLIC.pdf
Size:
1.82 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
SCIENCE