DETECTION OF ENTEROPATHOGENIC BACTERIA IN PUBLIC DRINKING WATER SOURCES IN SOME PARTS OF GUSAU MUNICIPAL
DETECTION OF ENTEROPATHOGENIC BACTERIA IN PUBLIC DRINKING WATER SOURCES IN SOME PARTS OF GUSAU MUNICIPAL
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Date
2007-12
Authors
GARBA, IBRAHIM
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Abstract
This study was aimed at determining the level of contamination, seasonal distribution as
well as the persistence of target organisms in well, tap and packaged water. A total of 180
well water samples, 60 tap water samples and 60 packaged water samples respectively
were obtained from some parts of Gusau Municipal, the samples were analysed using
standard procedures. Total coliform count, Heterotrophic plate count, some physicochemical
properties and presence of Escherichia coli, Salmonella species Vibro species
and Shigella species were determined for two seasons. All the water samples had coliform
and heterotrophic counts higher than the recommended standard for drinking water. The
number in both cases was higher in the wet season than the dry season. Biochemical and
serological identification showed that out of the 63 confirmed Escherichia coli isolates,
41 (45.4%) were from well water and of the 41 Salmonella species recovered, 31 (36.6%)
were from well water, while 20 ( 22.2%) Shigella species were also recovered from well
water. Pseudomonas species isolated from well water accounted for 44 (48.8%) of the
isolates while 22 (24.4%) Klebsiella species, 13 (14.3%) Proteus species and 38 (42.2%)
other unidentified isolates respectively. The tap water had 14 (23.3%) Escherichia coli, 9
(14.9%) Salmonella species, 12(20%) Shigella species and 32 (36.6%) Klebsiella species
while in contrast packaged water had 8 (13.3%) Escherichia coli, 2 (1.6%) Salmonella
species, 5 (8.3%) Shigella species and 14 (23.3%) Klebsiella species. No Vibrio species
were isolated in all cases. All the well waters failed to meet the minimum requirements
for nitrate and 5 day Biochemical Oxygen Demand. Result of susceptibility test showed
that Escherichia coli isolates were susceptible to Gentamycin, Pefloxacin,
Chloramphenicol, Tarivid, Augmentin, Streptomycin and Sprofloxacin , but resistance to
Amoxacilin was observed: Salmonella isolates showed susceptibility to Gentamycin,
Ciprofloxacin, Tarivid, Sparfloxacin, Amoxacilin, Streptomycin, Chloramphenicol and
Augementin, resistance to Septrin and Perfloxacin was however observed. The isolates ofShigella were susceptible to Perfloxacin, Streptomycin, Sprofloxacin, Tarivid, Amoxacin
and Augmentin. Adequate treatments of all waters, public health education and proper
antibiotics use are highly recommended.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL IN PARTIAL
FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE
DEGREE OF MASTER OF SCIENCE (MICROBIOLOGY)
DEPARTMENT OF MICROBIOLOGY
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY
ZARIA
DECEMBER, 2007
Keywords
DETECTION, ENTEROPATHOGENIC, BACTERIA, PUBLIC, DRINKING WATER, SOURCES, GUSAU MUNICIPAL