DETECTION OF ENTEROPATHOGENIC BACTERIA IN PUBLIC DRINKING WATER SOURCES IN SOME PARTS OF GUSAU MUNICIPAL

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Date
2007-12
Authors
GARBA, IBRAHIM
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Abstract
This study was aimed at determining the level of contamination, seasonal distribution as well as the persistence of target organisms in well, tap and packaged water. A total of 180 well water samples, 60 tap water samples and 60 packaged water samples respectively were obtained from some parts of Gusau Municipal, the samples were analysed using standard procedures. Total coliform count, Heterotrophic plate count, some physicochemical properties and presence of Escherichia coli, Salmonella species Vibro species and Shigella species were determined for two seasons. All the water samples had coliform and heterotrophic counts higher than the recommended standard for drinking water. The number in both cases was higher in the wet season than the dry season. Biochemical and serological identification showed that out of the 63 confirmed Escherichia coli isolates, 41 (45.4%) were from well water and of the 41 Salmonella species recovered, 31 (36.6%) were from well water, while 20 ( 22.2%) Shigella species were also recovered from well water. Pseudomonas species isolated from well water accounted for 44 (48.8%) of the isolates while 22 (24.4%) Klebsiella species, 13 (14.3%) Proteus species and 38 (42.2%) other unidentified isolates respectively. The tap water had 14 (23.3%) Escherichia coli, 9 (14.9%) Salmonella species, 12(20%) Shigella species and 32 (36.6%) Klebsiella species while in contrast packaged water had 8 (13.3%) Escherichia coli, 2 (1.6%) Salmonella species, 5 (8.3%) Shigella species and 14 (23.3%) Klebsiella species. No Vibrio species were isolated in all cases. All the well waters failed to meet the minimum requirements for nitrate and 5 day Biochemical Oxygen Demand. Result of susceptibility test showed that Escherichia coli isolates were susceptible to Gentamycin, Pefloxacin, Chloramphenicol, Tarivid, Augmentin, Streptomycin and Sprofloxacin , but resistance to Amoxacilin was observed: Salmonella isolates showed susceptibility to Gentamycin, Ciprofloxacin, Tarivid, Sparfloxacin, Amoxacilin, Streptomycin, Chloramphenicol and Augementin, resistance to Septrin and Perfloxacin was however observed. The isolates ofShigella were susceptible to Perfloxacin, Streptomycin, Sprofloxacin, Tarivid, Amoxacin and Augmentin. Adequate treatments of all waters, public health education and proper antibiotics use are highly recommended.
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A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE (MICROBIOLOGY) DEPARTMENT OF MICROBIOLOGY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY ZARIA DECEMBER, 2007
Keywords
DETECTION, ENTEROPATHOGENIC, BACTERIA, PUBLIC, DRINKING WATER, SOURCES, GUSAU MUNICIPAL
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