ISOLATION, ANITIBIOTIC AND HEAVY METAL SUSCEPTIBILITY PATTERNS OF SOME PATHOGENS FROM DOMESTIC DUMPSITES AND WASTE WATER
ISOLATION, ANITIBIOTIC AND HEAVY METAL SUSCEPTIBILITY PATTERNS OF SOME PATHOGENS FROM DOMESTIC DUMPSITES AND WASTE WATER
| dc.contributor.author | MZUNGU, IGNATIUS | |
| dc.date.accessioned | 2014-05-15T07:33:31Z | |
| dc.date.available | 2014-05-15T07:33:31Z | |
| dc.date.issued | 2007-11 | |
| dc.description | A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE (MICROBIOLOGY) DEPARTMENT OF MICROBIOLOGY AHMADU BELLO UNIVERSITY, ZARIA NIGERIA NOVEMBER, 2007 | en_US |
| dc.description.abstract | The impact of domestic wastes on the antibiotic susceptibility patterns of environmental pathogens was evaluated by isolating some pathogens from dumpsite soil and wastewater. All the isolates were screened for susceptibility to tetracycline (30μg), ofloxacin (30μg), gentamicin (10μg), contrimoxazole (25 μg), nitrofurantoin (300 μg), augmentin (30 μg), amoxicillin (25 μg) and nalidixic acid (30 μg), using the CILS disk diffusioin technique. Ten samples each of dumpsite and wastewater samples were also analyzed for the presence of heavy metal ions using the energy dispersive X-Ray Fluorescence (EDXRF). All isolates were also tested for heavy metal tolerance on Mueller-Hinton Agar supplemented with heavy metals, using the serial dilution technique. One hundred and eighty bacteria isolates obtained were characterized. These comprised Staphylococcus aureus (56), Escherichia coli (55), and Pseudomonas aeruginosa (69). At least 16(29%) of Staphylococcus aureus, 35(64%) of Escherichia coli and 59(86%) of Pseudomonas aeruginosa were resistant to at least two of the antibiotics tested. None of the Staphylococcus aureus was tolerant to any of the heavy metals tested at the highest concentration(s) used, but 17(31%) of Escherichia coli were tolerant to copper and 25(45%) were tolerant to cobalt, while 47(68%) of Pseudomonas aeruginosa were tolerant to at least two heavy metals. Correlation between metal tolerance and antibiotic resistance using χ2 test of association (P=0.05) showed (χ2 = 11.5). This suggests that, the presence of heavy metals in the environment may have constituted a selective pressure for multiple antibiotic resistance. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/4754 | |
| dc.language.iso | en | en_US |
| dc.subject | ISOLATION, | en_US |
| dc.subject | ANITIBIOTIC, | en_US |
| dc.subject | HEAVY METAL, | en_US |
| dc.subject | SUSCEPTIBILITY, | en_US |
| dc.subject | PATTERNS, | en_US |
| dc.subject | PATHOGENS, | en_US |
| dc.subject | DOMESTIC, | en_US |
| dc.subject | DUMPSITES, | en_US |
| dc.subject | WASTE WATER | en_US |
| dc.title | ISOLATION, ANITIBIOTIC AND HEAVY METAL SUSCEPTIBILITY PATTERNS OF SOME PATHOGENS FROM DOMESTIC DUMPSITES AND WASTE WATER | en_US |
| dc.type | Thesis | en_US |