MICROBIOLOGICAL ANALYSIS OF PACKAGED ORANGE DRINK MARKETED IN KADUNA METROPOLIS

Simple item page
dc.contributor.authorOLUSESAN, OLUTIMAYIN
dc.contributor.authorGBONJUBOLA
dc.date.accessioned2015-10-08T08:17:56Z
dc.date.available2015-10-08T08:17:56Z
dc.date.issued1995-03
dc.descriptionA THESIS SUBMITTED TO POSTGRADUATE SCHOOL AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILMENT OF THE RQUIREMENT FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN PHARMACEUTICAL MICROBIOLOGY DEPARTMENT OF PHAMACEUTICS & PHAMACEUTICAL MICROBIOLOGY FACULTY OF PHAMACEUTICAL SCIENCES AHMADU BELLO UNIVERSITY ZARIA, NIGERIA.en_US
dc.description.abstractThe microbiological quality of seven types of packaged orange drinks marketted in Kaduna metropolis were analysed by microbiological routine methods. The analysis involved the determination of the presence and the level of bacteria and fungi contaminants. The analysis was carried out under aseptic condition. All the orange drinks analysed had high level of microbial contamination. The bacteria count observed was between 3.0 x l(r to 1.43 x 106 cfu/ml, also the mould/yeast count was between 8.0 x 103 to 1.31 x 105 spu/ml. The bacteria species detected consisted of Escherichia coli, Pseudomonas aeruginosa. Micrococcus spp and the spore-forming Bacillus subtilis. The mould/yeast consisted mainly of Aspergillus niger, Penicillium sp, Rhizopus oryza, Candida guillermondi and Candida tropicalis. Orange drinks packaged in plastic bottles showed the highest bacteria count followed by those in glass bottles while those in foil paperboard showed the lowest bacteria count. However, drinks packaged in foiled paperboard showed the highest fungi count followed by those in glass bottle. E. coli and Ps. aeruginosa were less sensitive to sodium benzoate and benzoic acid used in the drinks as preservatives than B. subtilis and Micrococcus spp. Ps. aeruginosa appeared to be less sensitive to the preservatives than all the other isolates. Also, the isolates from the samples were found to be more sensitive than the standard cultures. The result of the Minimum Inhibitory Concentration (MIC) and Minimum Bacteriocidal Concentration (MBC) of the preservatives against the isolates and the standard cultures showed that the official recommendation of 0.1-0.5 ug/ml for their use looks relatively low. The measurement of surface charge of the isolates and the standard cultures showed that E. coli and Ps. aeruginosa isolated from the packaged orange drinks showed more absorption to the anion and cation resins than the standard NCTC cultures while the B. subtilis standard NCTC 8236 however showed more absorption than the isolated B. subtilis from the orange drinks. These differences in the surface properties may have direct effect on the sensitivity of these organisms to the preservatives. The result of this work has shown that the recommended concentration of the anti-microbial preservatives in the packaged orange drinks were not adequate. Also, there is need to adhere strictly to the Good Manufacturing Practice in the manufacturing companies.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6914
dc.language.isoenen_US
dc.subjectMICROBIOLOGICALen_US
dc.subjectANALYSISen_US
dc.subjectPACKAGEDen_US
dc.subjectORANGEen_US
dc.subjectDRINKen_US
dc.subjectMARKETEDen_US
dc.subjectKADUNAen_US
dc.titleMICROBIOLOGICAL ANALYSIS OF PACKAGED ORANGE DRINK MARKETED IN KADUNA METROPOLISen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
MICROBIOLOGICAL ANALYSIS OF PACKAGED ORANGE MARKETTED IN KADUNA METROPOLISpdf.pdf
Size:
4.35 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.58 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
SCIENCE