EFFECT OF SELENIUM ON IMMUNE RESPONSEAND SUSCEPTIBILITY OF VACCINATED COCKERELS TO INFECTIOUS BURSAL DISEASE

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dc.contributor.authorSHEKARO, Audu
dc.date.accessioned2021-06-17T08:29:22Z
dc.date.available2021-06-17T08:29:22Z
dc.date.issued2012-08
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN VETERINARY PATHOLOGY DEPARTMENT OF VETERINARY PATHOLOGY, FACULTY OF VETERINARY MEDICINE, AHMADU BELLO UNIVERSITY ZARIA, NIGERIAen_US
dc.description.abstractSelenium (Se) is a micronutrient that has been used to stimulate the immune system in animals. Infectious bursal disease (IBD) outbreaks in spite of vaccination are a common occurrence in poultry production in Nigeria. This study was conducted to determine the effect of Se supplementation on immune response and resistance of cockerels to IBD. In the experiment, 300 Dominant black marshal cockerels (DBMC) were divided into 12 groups of 25 birds each and fed graded levels of dietary Se. Groups A, B, C received 0.1 ppm, 0.5 ppm and 1.0 ppm of Se in feed, respectively. They were vaccinated with IBD vaccine (M.B strain) at 1 and 3 weeks old and challenged with IBD virus (IBDV) at 6 weeks old. Groups D, E, F received 0.1, 0.5 and 1.0 ppm of Se in feed, respectively; they did not received IBD vaccine, but were challenged at 6 weeks old with IBDV. Groups G, H, I received 0.1, 0.5 and 1.0 ppm of Se respectively, received IBD vaccine at 1 and 3 weeks old, but were not challenged with IBDV. Group J was not supplemented with Se but vaccinated with IBD vaccine at 1 and 3 weeks old but not challenged with IBDV. Group K was not supplemented with Se, not vaccinated with IBD vaccine and not challenged with IBDV. Group L did not receive Se supplementation, not vaccinated against IBD, but challenged with IBDV at six weeks old. Selenium concentration in the feed and tissues sample was determined using inductively coupled plasma optical spectroscopy. Antibody titre was determined using a commercial ELISA kit. Mortality and Protective rates in the challenged birds were calculated. Gross and microscopic lesions were scored. Haematological parameters were determined using auto haematology analyzer. Biuret method was used to determine total protein. After graded levels of Se supplementation for 10 weeks, there was no correlation between the dose of Se in feed and its concentration in the liver, muscles, blood and serum, but there was a positive correlation (r = 0.94) in the kidneys. Increase in dietary Se concentration led to increase in antibody titres. There was a negative correlation (r = - 0.93) in the vaccinated and (r = -0.95) in unvaccinated chicks between the concentration of Se in feed and mortality rate in chicks challenged with IBDV. There was a positive correlation between Se concentration in feed and haematological parameters. Lesions were severe in birds that received lower doses of Se than those that received higher doses. It was concluded that Se supplementation in feed enhances the immune response of DBMC to IBD and it also reduces the severity of the disease. Selenium supplementation in poultry feed was therefore recommended for industrial trial. .en_US
dc.identifier.urihttp://hdl.handle.net/123456789/12460
dc.language.isoenen_US
dc.subjectEFFECT,en_US
dc.subjectSELENIUM,en_US
dc.subjectIMMUNE RESPONSEAND SUSCEPTIBILITY,en_US
dc.subjectVACCINATED COCKERELS,en_US
dc.subjectINFECTIOUS BURSAL DISEASE.en_US
dc.titleEFFECT OF SELENIUM ON IMMUNE RESPONSEAND SUSCEPTIBILITY OF VACCINATED COCKERELS TO INFECTIOUS BURSAL DISEASEen_US
dc.typeThesisen_US
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