BIOETHANOL PRODUCTION FROM SUGARCANE BAGASSE AND RICE STALK USING LOCAL STRAINS OF ASPERGILLUS NIGERAN DSACCHAROMYCES CEREVISIAE AS CO-CULTURES
BIOETHANOL PRODUCTION FROM SUGARCANE BAGASSE AND RICE STALK USING LOCAL STRAINS OF ASPERGILLUS NIGERAN DSACCHAROMYCES CEREVISIAE AS CO-CULTURES
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Date
2017-10
Authors
ABUBAKAR, SALIM CHARANCHI
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Abstract
This study was aimed at producing an alternative energy source(bioethanol)from sulphuric acid and alkaline hydrogen peroxide treated sugarcane bagasse and rice stalkemploying simultaneous saccharification and fermentation process using co-cultures of isolated and characterized strains of Aspergillus niger and Saccharomyces cerevisiae. The proximate composition of the substrates was determine. The composition of sugarcane bagasse and rice stalk treated with sulphuric acid were determined to be (6.30% ash), (3.05% protein), (84.15% carbohydrates) and (4.75 ash), (5.25 protein), (80.55 carbohydrates) respectively, while that of sugarcane bagasse and rice stalk treated with alkaline peroxide were (7.95% ash), (1.75% protein), (83.20% carbohydrates) and (5.10% ash), (7.00% protein), (77.65% carbohydrates) respectively.The characterization scheme used were cultural, morphological and microscopic for A.nigerand cultural, microscopic and physiological for S. cerevisiae. Garden Soil(GS02) isolateof A. nigerthat possess 6.2mm and 17.1mmdiameter of zone of starch and cellulose hydrolysis respectively and palm wine (PW02) isolateof S. cerevisiaethat tolerates up to 15% ethanol where selected for the production. At optimal fermentation conditions of 35ºC temperature, 5.0 pH, 4% substrate concentration, 300rpm agitation rate and 4 days fermentation period, a maximum of 6.20g/100ml and 5.06 g/100ml of ethanol were obtained from sulphuric acid-treated sugarcane bagasse and rice stalk respectively.After determining the quantity of the ethanol produced using specific gravity method, qualitative analysis using FTIR-Spectrophotometrywhich showed the absorbance peaks of the ethanol functional groups of all the ethanol samples produced and the functional groups had their absorption peaks within their normal ranges of 3100-3600cm-1, 2800-3000cm-1 and 1600-1675cm-1 for hydroxyl functional group, saturated and unsaturated parts of the compound respectively.
In conclusion, 84.15% was the highest carbohydrate content observed, the isolates GS02 and PW02 were isolated, identified and employed for bioethanol production. The maximum quantity of ethanol obtained was 6.20g/100ml from sulphuric acid treated sugarcane bagasse and the absorption peaks of the functional groups were within their normal ranges
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
BIOETHANOL PRODUCTION,, SUGARCANE BAGASSE,, RICE STALK,, USING LOCAL STRAINS,, ASPERGILLUS NIGERAN DSACCHAROMYCES CEREVISIAE,, CO-CULTURES