EVALUATION OF PLATELET–DERIVED AND EPIDERMAL GROWTH FACTORS ON SURGICALLY- INDUCED WOUNDS IN NEW ZEALAND WHITE RABBITS TREATED WITH METHYLPREDNISOLONE

Simple item page
dc.contributor.authorJAPHETH, Joel Kalang
dc.date.accessioned2018-11-12T10:21:46Z
dc.date.available2018-11-12T10:21:46Z
dc.date.issued2017-11
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIAIN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY DEGREE IN VETERINARY SOFT-TISSUE SURGERY DEPARTMENT OF VETERINARY SURGERY AND RADIOLOGY, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe study was designed to evaluate the influence of platelet-derived growth factor (PDGF-AB) and epidermal growth factor (EGF-AB) on modulating wound healing of rabbits. A total of twenty four (24) New Zealand White rabbits of nine months to one year old from both sexes were used for the study. The rabbits weigh between 1.5–2.0 kg body weights. The rabbits were randomly grouped into two major groups (1 and 2) of twelve rabbits each. The rabbits were treated parenterally with methylprednisolone (methylP), and compared with the control treated parenterally with normal saline (NS). They were subsequently placed randomly, into four sub-groups each 1A (methylP + EGF), 1B (methylP + PDGF), 1C (methylP + NS) and 1D (methylP + EGF + PDGF); 2A (NS + EGF), 2B (NS + PDGF), 2C (NS + NS) and 2D (NS + EGF + PDGF) of three rabbits each. The animals were pre-anaesthetised with atropine sulphate and chlorpromazine. Ketamine hydrochloride was used for anaesthesia. An area of wound that is 2 cm × 1 cm dimension on rabbit dorsum, was created on each site of the dorsum. Group 1 were treated parenterally with methylP 0.5 mg/kg and the sub-groups were treated topically with 1A (EGF), 1B (PDGF), 1C (Normal saline) and 1D (EGF + PDGF). Group 2 were parenterally treated with normal saline and topically with 2A (EGF), 2B (PDGF), 2C (Normal saline) and 2D (EGF + PDGF). Each wound designated for PDGF, EGF and NS were smeared with 2.92 μg of PDGF, 20.8 μg of EGF, and 0.2 mL of normal saline, respectively. The wounds were monitored until complete healing. Doppler ultrasound of the scar tissue was carried out at the frequency of 5 Hz using C5 sonostar laptop ultrasound. Blood, serum and wound biopsy were taken. Wound retraction was recorded in all the groups with highest peak in NS + PDGF (3.13 cm2) at day 3 and 3.11 cm2 in NS + NS at day 5. Onset of epithelialisation was recorded at day 7 except for groups treated with EGF + PDGF the recorded epithelialisation at day 5 post-wounding. Inflammatory cells influx were diffused in the NS + NS and NS + EGF + PDGF groups. However, synergy in anti-inflammatory activity was not recorded with the use of methylP + PDGF but was observed on methylP + EGF. The PDGF group showed superior anti-inflammatory activity compared to the EGF treated rabbits. Ultrasonography of NS + NS scar line showed hyper-echogenicity with least vascular perfusion but small scar lines in the EGF and PDGF treated rabbits. The methylP + PDGF and methylP + EGF rabbits had the strongest scar strength as recorded by the tensile strength of 76.8 N/cm2 and 71.25 N/cm2, respectively. In conclusion, the overall healing time of methylprednisolone-treated rabbits was delayed; however the scar had the best connective tissue fibre arrangement. Reduced period of wound retraction was achieved with the use methylprednisolone, EGF or PDGF, thus, reduced wound surface area for contamination and reduced chances of wound infection. Smallest scar size was achieved with the use of PDGF and methylP + PDGF, thus, maybe important in cosmetic surgeries. The administration of methylprednisolone at 0.5mg/kg in wound management to enhance collagen fibre arrangements in scar tissue. Tensile strength was best observed in methylP + PDF and methylP + EGF, thus, are relevant in management of wounds in a weight carrying regions like the ventral aspects of the abdomen. The place of proper wound dressing in wound healing management cannot be replaced.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10854
dc.language.isoenen_US
dc.subjectEVALUATION,en_US
dc.subjectPLATELET–DERIVED,en_US
dc.subjectEPIDERMAL GROWTH,en_US
dc.subjectSURGICALLY- INDUCED WOUNDS,en_US
dc.subjectNEW ZEALAND,en_US
dc.subjectRABBITS TREATED,en_US
dc.subjectMETHYLPREDNISOLONEen_US
dc.titleEVALUATION OF PLATELET–DERIVED AND EPIDERMAL GROWTH FACTORS ON SURGICALLY- INDUCED WOUNDS IN NEW ZEALAND WHITE RABBITS TREATED WITH METHYLPREDNISOLONEen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
EVALUATION OF PLATELET–DERIVED AND EPIDERMAL GROWTH FACTORS ON SURGICALLY- INDUCED WOUNDS IN NEW ZEALAND WHITE RABBITS TREATED WITH METHYLPREDNISOLONE.pdf
Size:
3.08 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
VETERINARY MEDICINE