PESTICIDAL EFFECTS OF EXTRACTS FROM DIFFERENT PARTS OF NEEM PLANT {Azadirachta indica)

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Date
1998-03
Authors
SIKOD, RAYMOND DINGA
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Abstract
Various water dispersible powder (WDP) 5% concentration and dust preparation (DP) 5% concentration both based on crushed neem kernel and leaf, and an emulsifiable concentrate (EC) based on neem kernel oil were prepared. Their azadirachtin content were determined. These formulations (WDP and EC) were evaluated on the field against the two major pest of cowpea i.e. the leaf beetle Ootheca mutabilis (Sahl) and the pod sucking bug Clavigralla tomentosicollis (Stal) and in the laboratory (DP) against Callosobrachus maculatus (Fab) an economically important pest of stored cowpea grains. The various formulations were compared with each other and with a commercially available insecticide. In the field evaluation, the various formulations not only significantly (P < 0.05) reduced damage due to Ootheca mutabilis and increase grain yield in cowpea, but also gave a performance comparable to that of the synthetic insecticide (Cypermethrin). None of the formulations gave complete protection, but treatments with neem seed water extract (NSWE) and neem cake water extract (NCWE) resulted in less foliar damage and a lower incidence of Clavigralla tomentosicollis than the other formulations. In most instances, untreated plots were severely damaged whereas those protected with various neem extracts were not attacked or only slightly damaged. This was confirmed by a strong and significant (P < 0.05) correlation between azadirachtin and pesticidal effects (r = - 0.96, .86, -.97, -0.6 and -0.6) for leaf damage, yield, insect population, seed damage and weight loss respectively. However, there were significant (P < 0.05) variations among the treatments. Laboratory trials for post-harvest storage indicated that, grains treated with various neem dust preparation (DP) and neem oil (4ml/kg) caused significant (P < 0.05) reduction in number of progeny and survival rate of Callosobrachus maculatus. There was no adult survivors or progeny production in grains treated with neem oil. Neem oil and neem cake protected the grains against feeding by Callosobrachus maculatus resulting in lower percentage weight loss and damage seeds compared to untreated grains and the standard actellic dust 16 weeks after infestation. The germinative percentage of seed treated with these materials indicated that, they do not impair viability and hence could be used in preserving grains as well as seeds for planting.
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A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE (M.Sc) DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.
Keywords
PESTICIDAL,, EFFECTS,, EXTRACTS,, DIFFERENT,, PARTS,, NEEM,, PLANT,, Azadirachta indica
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