ASSESSMENT OF POST- CASTRATION PAIN IN RABBITS TREATED WITH THE ETHANOL EXTRACT OF CHENOPODIUM AMBROSIOIDES LINN

Simple item page
dc.contributor.authorOLADIMEJI,lateefa opeyemi
dc.date.accessioned2019-04-26T13:43:52Z
dc.date.available2019-04-26T13:43:52Z
dc.date.issued2018-10
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE IN FOOD ANIMAL SURGERY DEPARTMENT OF VETERINARY SURGERY AND RADIOLOGY, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThis study was carried out to assess post-castration pain and distress using blood glucose concentration; oxidative stress parameters, algometer, and rabbit grimace scale in rabbits treated with the ethanol extract of Chenopodium ambrosioides (C. ambrosioides). Fifteen (15) sexually matured and clinically healthy bucks with the mean weight of about 2.5 ± 0.7 kg were used for the study. The animals were allowed to acclimatize to the management conditions for two weeks. The research was carried out in two phases: Phase 1 comprised three groups, A, B and C that contained 3 rabbits each, and were treated orally with normal saline, meloxicam tablet (0.1 mg/kg), and C. ambrosioides extract (500 mg/kg), respectively after castration. The phase 2 contained groups D and E with 3 animals each, and were treated topically with Fastum gel® (Ketoprofen 2.5 %) and C. ambrosioides (applied liberally) extract after castration. Two milliliter (2 ml) of blood sample was collected from each buck to assay for Malondialdehyde (MDA) concentration, Superoxide Dismutase (SOD), and Glutathione peroxidase (GPx) activities, 1 hr, 2 hr, and 3 hr post treatment, following the evaluation of blood glucose concentration. Post-castration pain assessment was done using the Rabbit Grimace scale at 1 hr post treatment for twenty minutes followed by post-castration pain evaluation using algometer around the surgical wound. The pain evaluation using algometer was also repeated on the day one of surgery at 1hr post treatment. Fifteen minutes after the treatments on day zero of surgery, the blood glucose concentration (mg/dL) of the normal saline treatment group (267.67 ± 18.77 mg/dL) was significantly higher (p ≤ 0.05) than all other treatment groups. At two hours after treatment, the plasma MDA concentration (μmol/L) of the normal saline treatment group (44696 ± 19071 μmol/L) was significantly higher (p ≤ 0.05) than all other treatment groups. The plasma SOD activity of oral C. ambrosioides (22 ± 5.7 mg/mL) treatment was higher than the oral meloxicam (14 ± 6 mg/mL) and normal saline (18 ± 8.4 mg/mL) treatments, while that of topical C. ambrosioides (4.8 ± 1.1 mg/mL) treatment group was higher than that of topical Ketoprofen (2.2 ± 1 mg/mL) treatment, though the difference was not significant. The force required in eliciting pain after 1 hour in topical C. ambrosioides (16.03 ± 2.52N) treatment group was significantly higher than the topical Ketoprofen (10.27 ± 0.17 N) treatment group. While the oral C. ambrosioides (13.47 ± 1.32N) treatment was higher than the oral meloxicam (12.9 ± 1.42 N) and normal saline (9.87 ± 0.35 N) treatment groups though, the differences were not significant. The overall grimace pain score for the oral meloxicam (0.51 ± 0-0.9), oral C. ambrosioides (0.71 ± 0.1-1.4), topical C. ambrosioides (0.55 ± 0.3-1.2) extracts treatment groups was not different from that of the pre-surgical grimace pain score (0.36 ± 0-1), while that of the topical Ketoprofen score (0.8 ± 0.4-1.7) and oral C. ambrosioides (0.71 ± 0.1-1.4) extracts were not different from the normal saline (0.92 ± 0.5-1.6) treatment group. Chenopodium ambrosioides had better antioxidative effects when administered orally, while the topical application of C. ambrosioides extracts produced faster analgesic effects compared to the oral administration at the dosage used. This study has demonstrated that C. ambrosioides could be used to reduce post-castration pain and distress in rabbits as evaluated using oxidative stress parameters, algometer and rabbit grimace scale.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/11572
dc.language.isoenen_US
dc.subjectASSESSMENT,en_US
dc.subjectPOST- CASTRATION PAIN,en_US
dc.subjectRABBITS TREATED,en_US
dc.subjectETHANOL EXTRACT,en_US
dc.subjectCHENOPODIUM AMBROSIOIDES LINN,en_US
dc.titleASSESSMENT OF POST- CASTRATION PAIN IN RABBITS TREATED WITH THE ETHANOL EXTRACT OF CHENOPODIUM AMBROSIOIDES LINNen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ASSESSMENT OF POST- CASTRATION PAIN IN RABBITS TREATED WITH THE ETHANOL.pdf
Size:
2.32 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
VETERINARY MEDICINE