EFFECTS OF PETROLEUM ETHER AND METHANOLIC FRACTIONS OF AVOCADO PEAR (PERSEA AMERICANA MILL) SEEDS ON WISTAR RATS FED A HIGH FAT-HIGH CHOLESTEROL DIET

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dc.contributor.authorAFAHAKAN, MFONOBONG EFIONG
dc.date.accessioned2014-02-25T09:05:41Z
dc.date.available2014-02-25T09:05:41Z
dc.date.issued2012-12
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY ZARIA, NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY ZARIA, NIGERIA DECEMBER, 201en_US
dc.description.abstractThe effect of petroleum ether and methanolic fractions of Avocado pear (Persea americana Mill) in diet-induced hyperlipidemic rats was studied. Eleven groups of five rats each were treated for four weeks and they included normal control, the high fat high cholesterol diet (HFD) control group, HFD + 0.20mg/kgbw Atorvastatin, and groups treated with 125mg/kg, 250mg/kg and 500mg/kg body weight petroleum ether and methanolic fractions. Quantitative phytochemicals analysis revealed significantly (p<0.05) higher quantities of saponins (10.30±0.60%), flavonoids (74.30±5.20%), and phenols (27.83±2.47%) in the methanolic fraction. Result of biochemical analysis showed HFD control group had significant (p<0.05) increases in the serum total cholesterol (TC), low density lipoprotein cholesterol (LDL-c), triacylglycerols, total lipids, total phospholipids, and the atherogenic index (LDL-c/HDL-c and TC/HDL-c) (268.60±30.40mg/dl, 233.40±30.5mg/dl, 71.70±4.30mg/dl, 13.10±3.00g/dl, 5.70±0.90g/dl, 13.00±1.90, 10.00±3.50 respectively); while it recorded significant (p<0.05) decrease in high density lipoprotein cholesterol (HDL-c) (20.70±4.30mg/dl). These effects were reversed in the extracts treated groups. There was a significant (p<0.05) increase in thiobarbituric reactive substances (4.73±1.50μmol (NC), and 24.98±5.90μmol (HFD) respectively) and significant (p<0.05) decrease in organ catalase (0.04±0.01mol/min/g (NC) and 0.02±0.01mol/min/g (HFD) respectively) and superoxide dismutase activities (152.00±19.10unit/g (NC) and 139.90±8.80unit/g (HFD) respectively) observed in the liver and kidney homogenate in the HFD control group which were reversed in the extracts treated groups. There was no significant (p>0.05) difference in the level of reduced gluthathione in both the liver and kidney homogenate in normal and HFD control groups. Serum alanine and aspartate aminotransferases showed no significant (p>0.05) differences between the normal and HFD control groups, while there was significant (p<0.05) decrease in alkaline phosphatase level. The serum urea level decrease (p<0.05) significantly in the treatment groups when compared with the normal control group. The petroleum ether and methanolic fractions of Persea americana seed has been shown to have hypolipidemic and hypocholesterolemic potentials as well as in vivo antioxidant activity in dietinduced hyperlipidemic rats.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2477
dc.language.isoenen_US
dc.subjectEFFECTSen_US
dc.subjectPETROLEUM ETHERen_US
dc.subjectMETHANOLIC FRACTIONSen_US
dc.subjectSEEDSen_US
dc.subjectAVOCADO PEAR (PERSEA AMERICANA MILL)en_US
dc.subjectWISTAR RATSen_US
dc.subjectHIGH FAT-HIGH CHOLESTEROL DIET BYen_US
dc.titleEFFECTS OF PETROLEUM ETHER AND METHANOLIC FRACTIONS OF AVOCADO PEAR (PERSEA AMERICANA MILL) SEEDS ON WISTAR RATS FED A HIGH FAT-HIGH CHOLESTEROL DIETen_US
dc.typeThesisen_US
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