MOLECULAR DETECTION OF GENETIC MARKERS ASSOCIATED WITH PLASMODIUM FALCIPARUMDRUG RESISTANCE AMONG PATIENTS ATTENDING SOME HOSPITALS IN PARTS OF KADUNA STATE, NIGERIA

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dc.contributor.authorBENJAMIN, Gideon Yakusak
dc.date.accessioned2021-11-04T07:52:15Z
dc.date.available2021-11-04T07:52:15Z
dc.date.issued2021-04
dc.descriptionA DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF LIFE SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractAntimalarial drug resistance is a major obstacle to management and control of malariain Nigeria and sub-Saharan Africa. This research was aimed at carrying out molecular detection of genetic markers associated with Plasmodium falciparum specificdrug resistance among patients attending some hospitals in parts of Kaduna state, Nigeria.Three hundred (300) blood samples were collected from consenting individuals attending selected hospitals, in the three senatorial districts of Kaduna State, Nigeria. The samples were screened for malaria parasites by microscopy and CareStartTMmalaria rapid diagnostic test kit. Structured questionnaire were used to obtain bio-data and demographic data from the study participants. The data obtained were analysed using Statistical Package for Social Sciences (SPSS) and Chi square to check for association. Out of the 300 blood samples screenedby microscopy and Malaria Rapid Diagnostic Test (MRDT); 71 (23.7%) were positive by microscopy while 65 (21.7%) were positive by MRDT and only Plasmodium falciparum was detected. Patients attending General hospital Kafanchan had the highest occurrence (30%) of Plasmodium falciparum infection. The age group ≤10 had the highest prevalence of malaria (p<0.05). Married participants had higher prevalence (31.8%) of malaria than participants who were single (16%)or divorced (12.5%)(p<0.05). Those who use insecticide spray at home had lower prevalence (20.0%) of malaria compared to those who do not (p<0.05). Of all the malaria positive participants, those with Haemoglobin AA (73%) haemoglobingenotype had the highest percentage followed by Haemoglobin AS (23%), Haemoglobin AC (3%) and HaemoglobinSS (1).Plasmodiumfalciparumchloroquineresistance transporter (pfcrt), Plasmodiumfalciparum multidrug resistance 1 (pfmdr1), Plasmodium falciparum viii dihydrofolatereductase (pfdhfr), Plasmodium falciparum dihydropteroate synthase (pfdhps)genes were detected at expected amplicon sizes from the malaria positive samples in this study. Pfcrt (80%) had the highest prevalence, followed by pfdhfr (60%), pfmdr1 (36%) and pfdhps (8%).The phylogenetic tree showed that pfatpase6sequences in this study were related to published sequences. The findings of the study revealed association between malaria and age, and marital statusof participants. Study also demonstrated the presence of pfcrt, pfmdr1, pfdhfr, pfdhpsand pfatpase6which are associated with antimalarial resistance.DNA sequencing and bioinformatics tools such as sequence alignment and BLASTcontributed to proper identification and confirmation of genes after PCR and gel electrophoresis.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/12696
dc.language.isoenen_US
dc.subjectMOLECULAR DETECTION,en_US
dc.subjectGENETIC MARKERS ASSOCIATED,en_US
dc.subjectPLASMODIUM FALCIPARUMDRUG RESISTANCE,en_US
dc.subjectPATIENTS ATTENDING,en_US
dc.subjectHOSPITALS,en_US
dc.subjectPARTS,en_US
dc.subjectKADUNA STATE,en_US
dc.subjectNIGERIAen_US
dc.titleMOLECULAR DETECTION OF GENETIC MARKERS ASSOCIATED WITH PLASMODIUM FALCIPARUMDRUG RESISTANCE AMONG PATIENTS ATTENDING SOME HOSPITALS IN PARTS OF KADUNA STATE, NIGERIAen_US
dc.typeThesisen_US
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