PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ANALYSIS OF THE CRUDE EXTRACTS OF THE AERIAL PART OF AESCHYNOMENE UNIFLORA .MEY (FABACEAE)
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ANALYSIS OF THE CRUDE EXTRACTS OF THE AERIAL PART OF AESCHYNOMENE UNIFLORA .MEY (FABACEAE)
No Thumbnail Available
Date
2014-06
Authors
ACHIKA, JONATHAN ILEMONA
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The preliminary phytochemical screening of the aerial parts of Aeschynomene uniflora was carried out using standard method. The result of the phytochemical screening of crude petroleum ether, chloroform, ethyl acetate and methanol extracts revealed the presence of carbohydrate, cardiac glycoside, tannins, saponins flavonoids and anthraquinone The antimicrobial screening against Corynbacterium ulcerans, Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Candida albicans, Candida stellatoidea and Candida krusei was done using agar well diffusion method. The zone of inhibition of growth of microorganisms ranged from 16 - 18 mm for the petroleum ether extract, 22-27 mm for the chloroform extract, 22-26 mm for ethyl acetate extract and 19-22 mm for the methanol extract. The minimum inhibitory concentrations of the extracts were found to be between 15 mg/ml and 30 mg/ml while the minimum bactericidal /fungicidal concentration were found to be between 15 mg/ml and 60 mg/ml. The purification of the n-hexane fraction of the extract of Aeschynomene uniflora using a column of silica gel gave 3β, 22E-Stigmasta-5, 22-dien-3-ol (C29H48O, 417 g/mol) (stigmasterol). The structure of the isolated compound was established based on spectral data (IR, 1D- and 2D- NMR) and comparison with literature. The compound is reported for the first time in this plant.
8
Table of content Certification ii Declaration ii Dedication iii Acknowledgement iv Abstract v Table of content vi List of Tables xii List of Figures xiii Abbreviation xix 1.0 INTRODUCTION 1 1.1 Biological Background of Medicinal Plants 2 1.2 History of Herbal Medicine 4 1.2.1 Herbal medicine today 5 1.3 Justification of the Research 7 1.4 Scope and Limitation of This Study 8
1.5 Aims
Description
A THESIS SUBMITTED TO THE SCHOOL POST GRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF A MASTERS DEGREE IN CHEMISTRY DEPARTMENT OF CHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA NIGERIA
Keywords
PHYTOCHEMICAL SCREENING,, ANTIMICROBIAL ANALYSIS,, CRUDE EXTRACTS,, AERIAL,, AESCHYNOMENE,, UNIFLORA,, MEY,