ANTIMALARIAL POTENTIAL OF LEAF AND STEM BARK FRACTIONS OF METHANOL EXTRACT OF DIOSPYROS MESPILIFORMIS ON PLASMODIUM BERGHEI (NK 65) INFECTED MICE
ANTIMALARIAL POTENTIAL OF LEAF AND STEM BARK FRACTIONS OF METHANOL EXTRACT OF DIOSPYROS MESPILIFORMIS ON PLASMODIUM BERGHEI (NK 65) INFECTED MICE
No Thumbnail Available
Date
2016-01
Authors
OGUCHE, MERCY
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Antimalarial potential of various fractions of the methanolic extract of the leaf and stem bark of Diospyros mespiliformis on Plasmodium berghei (NK-65) infected mice and the inhibitory effect of the extracts on cysteine protease of the parasite were investigated. The oral median lethal dose (LD50) of the leaf and stem bark extracts were determined to be 400 and 200mg/kg body weight respectively. At the end of the four (4) days suppressive test,the effect of the fractions of leaf and stem bark methanol extracts on parasitaemia suppressive activity were dose-dependent for various fractions and n- butanol fraction having the highest suppressive activity. The effect of the leaf methanol extract on Liver marker enzymes (Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT)) showed significant (P<0.05) increase in the n-butanol and aqueous fractions and significant (P<0.05) increase in the serum level of (Alkaline phosphatase (ALP)) of the infected group but the stem bark extract showed significant (P<0.05) increase in the infected group of all the fractions and significant (P<0.05) decrease in the infected but treated with low and high dose of the extract. Packed cell volume and Haemoglobin concentration showed significant (P<0.05) decrease in the infected group compared to normal control and infected treated group. The enzyme was purified to 12.25-fold and a yield of 35.69% on Sephadex G-50 column with a final specific activity of 47.30μmol/min. The inhibitory effect of the bioactive fractions of (leaf and stem bark), on the enzyme (cysteine protease) was dose dependent in which the leaf and stem bark extracts exhibit mixed non-competitive and competitive pattern of inhibition respectively. These compounds could serve as a good source of antidote for malaria and could as well guide the design of a novel drug without side effects.
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL, AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE (M.Sc) IN BIOCHEMISTRY DEPARTMENT OF BIOCHEMISTRY FACULTY OF SCIENCE AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
ANTIMALARIAL POTENTIAL,, LEAF,, STEM BARK FRACTIONS,, METHANOL EXTRACT,, DIOSPYROS MESPILIFORMIS,, PLASMODIUM BERGHEI,, (NK 65),, INFECTED MICE,