ANTIMICROBIAL ACTIVITY OF METHANOLIC AND AQUEOUS EXTRACTS AND AMINO ACID PROFILE OF THE PARTIALLY PURIFIED PROTEIN OF GUIERA SENEGALENSIS (MOSHI MEDICINE)
ANTIMICROBIAL ACTIVITY OF METHANOLIC AND AQUEOUS EXTRACTS AND AMINO ACID PROFILE OF THE PARTIALLY PURIFIED PROTEIN OF GUIERA SENEGALENSIS (MOSHI MEDICINE)
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Date
2015-10
Authors
JIYIL, KIRWE MARKUS
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Abstract
Microorganisms have evolved defence mechanisms against antimicrobial agents and are resistant to some antibiotics.This study was aimed at evaluating the antimicrobial activity of methanolic and aqueous extracts and amino acid profile of the partially purified protein of Guiera senegalensis (moshi medicine). The antimicrobial activities of the extracts were assayed by agar disc diffusion and nutrients broth dilution techniques. Antimicrobial activity showed that, all the extracts were active against most of the isolates except Candida albican (fungus). Aqueous extract of matured and young leaves showed zones of inhibition ranging from 11.00 -26.00mm while aqueous extract of matured and young roots showed zones of inhibitions ranging from 11.00 – 19.00mm. Methanolic of matured and young leaves showed zones of inhibition ranging from 11.00 – 42.00mm while matured and young roots ranging from 11.00 – 37.00mm. The crude proteins were active against gram positive bacteria. Maximum zone of inhibition (42.00±1.00mm) was observed in methanolic extracts of young leaves against Staphylococcus aureus at 100mg/ml. Most extracts exhibited minimum inhibitory concentration (MIC) at range of 6.25mg/ml and 12.5mg/ml and MBC at 12.5mg/ml and 25mg/ml. The methanolic extract was observed to be more potent than the aqueous extract. The young leaves and roots were more active than matured leaves and roots of the plant. Seventeen (17) amino acids were quantified, indicating high Concentration in young leaves than matured leaves and roots. Glutamic acids and Aspartic acid were found in higher concentration in both leaves and roots of the plant. The molecular weight of the partial purified proteins of the matured leaves were 25.67 kDa and 149.2 kDa at protein concentrations of 1.10mg/ml while the young leaves were 20.33 kDa and 45.50 kDa at protein concentration of 1.20mg/ml. The presence of bioactive secondary metabolites, antimicrobial amino acids, low minimum inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) justifies the traditional uses of the leaves and roots of Guiera senegalensis for therapeutic purposes.
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A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFIlLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER DEGREE IN BIOCHEMISTRY.
DEPARTMENT OF BIOCHEMISTRY,
FACULTY OF SCIENCE
AHMADU BELLO UNIVERSITY, ZARIA
NIGERIA.
Keywords
ANTIMICROBIAL ACTIVITY,, METHANOLIC,, AQUEOUS EXTRACTS,, AMINO ACID PROFILE,, PARTIALLY PURIFIED PROTEIN,, GUIERA SENEGALENSIS,, (MOSHI MEDICINE),