EFFECTS OF FRACTIONSFROM CRUDE METHANOLIC EXTRACT OFAZADIRACHTA INDICA (NEEM)LEAF ON SPERMIOGRAM, TESTOSTERONE PROFILE, TESTES HISTOLOGY AND HAEMATOLOGYOF NEW ZEALAND WHITE RABBIT BUCKS

Simple item page
dc.contributor.authorUMAR, MOHAMMED SAIFULLAH
dc.date.accessioned2021-09-15T15:42:10Z
dc.date.available2021-09-15T15:42:10Z
dc.date.issued2021-02
dc.descriptionA THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN THERIOGENOLOGY DEPARTMENT OF THERIOGENOLOGY AND PRODUCTION, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIAen_US
dc.description.abstractThe effects of different fractionsfrom methanolic extract of Azadirachta indica (neems) leaves on spermatogenesis, testicular histology, testosterone concentration and haematological parameters in New Zealand white rabbits were evaluated in this study. Crude extract of the powdered leaves of neem (Azadirachta Indica) was extracted using methanol and further fractionated using hexane, chloroform, ethylacetate and butanol. Twenty five matured New Zealand white rabbit bucks wererandomly assigned to five groups (A, B, C, D, and E). Group A rabbits served as control and were administered only distilled water, while groups B, C, D and E, rabbits representing the hexane, chloroform, ethylacetate and butanol groups, respectively, were administered fractions from the respective solventextracts at similar dose rate of 300mg/kg. Semen samples were collected using artificial vagina weekly for fourteen weeks and were immediately evaluated for parameters such as volume, colour, motility, concentration, and percentage live ratio. Five millilitres of blood sample was collected from each rabbit buck through venipuncture of the ear vein three times during the experimental period, that was at the beginning, middle and end of the experiment for determination of haematological parameters. Serum sample was also harvested from the collected blood for determination of testosterone concentration. Two rabbit bucks from each group were humanely sacrificed at the end of the experiment for testicular histology. Data collected were analysed using graph pad prism software version 5.0 and P values, ≤0.05 were considered statically significant between treatment groups and the control. No significant difference (P≥0.05) was observed in mean sperm motility between the control group (90.00±2.04%) and treated groups B (84.33±5.36%), D (90.00±4.40%) and E (95.00±0.00%) at first week. However, significant (P<0.05) decrease in sperm motility was recorded from the treatment group C that receive chloroform fraction (00.68±1.64%) when compared with the control group (90.00±2.04%) at the end of the experiment.Although, no significance differences (p>0.05) were observed in semen volume between the treatment groups and the control group. However, numerical decrease in semen volume that is not significant (P>0.05) was observed in group C which was treated with chloroform fraction. Significant difference (P<0.05) in sperm concentration and sperm abnormalities were observed between the control group and the treatment groups. No significant (P>0.05) difference were observed in testosterone concentration between the various treatment group and the control group, however, there was gradual decrease in plasma concentration of testosterone values in the treatment groups when compared with the control group. Significant differences (P<0.05) in haemoglobin concentration, packed cell volume, red and white blood cell counts were observed between the treatment groups and the control. There was a significant decrease (P<0.05) in red blood cell count in the treatment groups; hexane fraction (5.70±0.07×1012/L), Chloroform extract (5.28±0.02×1012/L), ethylacetate fraction (5.52±0.00×1012/L) and butanol fraction (4.81±0.00×1012/L) compared with the control (6.54±0.00×1012/L), however, the values are within the normal haematological range values for the New Zealand white rabbit. Testicular degeneration, depletion and vacuolation of spermatogenic cell layers were observed in treatment group with chloroform fraction. However, other treated groups did not show difference from the control with intact and prominent seminiferous tubules and spermatogenic layers at different stages of division. Further studies need to be conducted to validate this findings and also to isolate specific antifertility agent(s) from chloroform fraction. The study showed that chloroform fraction of crude methanolic Azadirachta indica (neem) leaf extract was detrimental to spermatogenesis, which significantly reduced gross semen motility and testicular histology, while butanol, ethyl acetate, and hexane fractions had no significant effect on semen characteristics, histology and androgen profile of New Zealand white rabbit bucks.All the fractions from crude methanol neem leaf extract significantly reduce the PCV and RBC count but had no significant effect on testosterone concentration in the New Zealand white rabbit bucksen_US
dc.identifier.urihttp://hdl.handle.net/123456789/12633
dc.language.isoenen_US
dc.subjectEFFECTS OF FACTIONS,en_US
dc.subjectCRUDE METHANOLIC EXTRACT OFAZADIRACHTA INDICA,en_US
dc.subjectLEAF ON SPERMIOGRAM,en_US
dc.subjectTESTOSTERONE PROFILE,en_US
dc.subjectTESTES HISTOLOGY AND HAEMATOLOGY,en_US
dc.subjectNEW ZEALAND WHITE RABBIT BUCKSen_US
dc.titleEFFECTS OF FRACTIONSFROM CRUDE METHANOLIC EXTRACT OFAZADIRACHTA INDICA (NEEM)LEAF ON SPERMIOGRAM, TESTOSTERONE PROFILE, TESTES HISTOLOGY AND HAEMATOLOGYOF NEW ZEALAND WHITE RABBIT BUCKSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
EFFECTS OF FRACTIONSFROM CRUDE METHANOLIC EXTRACT .pdf
Size:
1.48 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
VETERINARY MEDICINE