ANTIBIOTICS SUSCEPTIBILITY STUDIES OF SOME BACTERIAL ISOLATES FROMPACKAGED MILK MARKETED IN ZARIA, NIGERIA
ANTIBIOTICS SUSCEPTIBILITY STUDIES OF SOME BACTERIAL ISOLATES FROMPACKAGED MILK MARKETED IN ZARIA, NIGERIA
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Date
2012-11
Authors
DAMIAN, UMOFIA INIMFON.
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Abstract
ABSTRACT
Milk contamination with antibiotic resistant bacteria can be a major threat to public health, as the
antibiotic resistant determinants can be transferred to other pathogenic bacteria potentially
compromising the treatment of severe bacterial infections. This study was conducted to
investigate the antibiotics susceptibility of bacterial isolates frompackaged milks marketed in
Zaria. Two hundred packaged milk samples were bought from five locations (forty samples from
each) in Zaria. Isolation and identification of the bacteria specieswere carried out using standard
microbiological procedures. Antibiotics susceptibility of the isolates was determined using a
panel of 12 antibiotics by disc diffusion method following Clinical Laboratory Standard Institute
guidelines. Minimum Inhibitory Concentration (M.I.C.)was determined using agar plate dilution
method.Conjugative studies were carried out with multiple antibiotics resistant isolates from
milk samples. The resistant isolates were subjected to DNA isolation and agarose gel
electrophoresis. The result obtained showed that the major contaminants of milk products
analysed were Pseudomonas sppclosely followed by Enterobactersppand Escherichia coli and
the overall contamination level of bacterial isolates in this study was 76.5%. One hundred and
fifty-three bacterial isolates were identified from the milk sample, 27.5% were obtained from the
first brand of milk sample, 21.6% from the second brand, 12.4% from the third brand and 38.6%
from the fourth brand of milk samples. Susceptibility result showedthat high percentage of
isolates were resistant to cloxacillin (99.35%), erythromycin (98%), amoxicillin (83.01%),
chloramphenicol (83%) and tetracycline (81.7%) but were however susceptible to ofloxacin
(99.3%) and gentamicin (83%). Multiple antibiotics resistance indices (MARI) showed that
bacterial isolates from the studied packaged milk samples were multi-resistant with MARI
ranging from 0.2 to 1.0. Out of ninety enterobacteriaceae studied, 93.3% of the bacterial isolates
had MAR index of 0.3 and above.Conjugation studies revealed that nineteen out of twenty-six.
Description
DEPARTMENT OF PHARMACEUTICS AND PHARMACEUTICAL
MICROBIOLOGY, FACULTY OF PHARMACEUTICAL SCIENCES
AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.
Keywords
ANTIBIOTICS,, SUSCEPTIBILITY,, STUDIES,, BACTERIAL,, ISOLATES,, FROMPACKAGED,, MILK,, MARKETED,, ZARIA,, NIGERIA.