CO-INFECTION OF SCHISTOSOMA HAEMATOBIUM AND ESCHERICHIA COLI IN PUPILS ATTENDING SOME SELECTED SCHOOLS IN ZARIA, NIGERIA
CO-INFECTION OF SCHISTOSOMA HAEMATOBIUM AND ESCHERICHIA COLI IN PUPILS ATTENDING SOME SELECTED SCHOOLS IN ZARIA, NIGERIA
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Date
2017-03
Authors
BALOGUN, ADINOYI DAVID
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Abstract
This study was designed to investigate co-infection of Schistosoma haematobium and Escherichia coli in pupils attending some selected primary schools in Zaria, Nigeria. A total of 520 urine samples were collected from the pupils and were analyzed for ova of Schistosoma haematobium and also the urines were also cultured for Escherichia coli. The ova of Schsitosomiasis was determined using the standard centrifugation method. E,coli was isolated using Cystiene Lactose Electrolyte Deficient (CLED) Agar and was subcultured on Eosin Methylene Blue(EMB) Agar. IMVIC biochemical test were carried out on the isolates. Further biochemical test was carried out to confirm the isolates were Escherichia coli. Antibiotic susceptibility test was carried out on the E,coli isolate using disk diffusion method. The prevalence of Schistosoma haematobium was 20.4%, Escherichia coli was 4.6% and the co-infection was 4.6%. The prevalence rates of Schistosoma haematobium infection in LEA Z, LEA J, LEA G, and LEA P were 14.6%, 26.9%, 18.5% and 21.5% respectively. The prevalence of E. coli and co-infection in LEA Z, LEA J, LEA G and LEA P were 2.3%. 5.4%, 4.6% and 6.2%. The highest prevalence of Schistosoma haematobium was found among the age group of 10-14years There was a significant association (χ2=8.936, df=3, P=0.030*) between age and Schistosoma haematobium infection. The prevalence of E.coli was found to be higher among the age group of 15-19 years and the prevalence was higher in the female (4.7%) than male (4.6%). There was no significant association between E. coli infection and age. The prevalence of Schistosoma haematobium in male pupils (23.5%) was found to be higher than the female pupils (15.0%). There was significant association between Schistosoma haematobium and Gender. The prevalence of Escherichia coli in females pupils was higher when compared to the male pupils. There was no significant association between E. coli and gender. Higher prevalence of
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Schistosoma haematobium was found among pupils who use streams /lakes as source of drinking water. There was significant association between S. haematobium and source of drinking water.The antibacterial susceptibility profile of the isolates was determined using 10 different antibiotics. All the isolates were susceptible to Gentamicin. Some of the isolates were resistant to Ciprofloxacillin, Amoxycillin, Chloramphenicol, Tetracycline, Sulphamethoxazole Trimethropin, Nalixidic acid, Ampicillin, Doxycycline and Nitrofurantoin
Description
A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL,
AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTER OF SCIENCE DEGREE IN MICROBIOLOGY
DEPARTMENT OF MICROBIOLOGY,
FACULTY OF LIFE SCIENCES,
AHMADU BELLO UNIVERSITY,
ZARIA, NIGERIA
Keywords
CO-INFECTION,, SCHISTOSOMA HAEMATOBIUM,, ESCHERICHIA COLI,, PUPILS ATTENDING,, SELECTED SCHOOLS,, ZARIA,, NIGERIA