SCIENCE

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Browsing SCIENCE by Subject "(ALBINO RATS),"

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    ANTITRYPANOSOMAL EFFECTS OF BRASSICA OLERACEA (CABBAGE) METHANOLIC AND AQUEOUS EXTRACTS ON RATTUS NORVEGICUS (ALBINO RATS) INFECTED WITH TRYPANOSOMA EVANSI
    (2014-11) MOH, Kingsley Onyekachi
    The toxicity of synthetic drugs commonly used in the treatment of trypanosomiasis has become a problem in livestock management and there is an urgent need to provide a safer and a biological alternative in the treatment of the disease. Methanolic and aqueous extracts of Brassica oleracea were assessed for their antitrypanosomal effects on Trypanosoma evansi in laboratory rats. The phytochemical analysis of the plant extracts revealed the presence of carbohydrates, cardiac glycosides, unsaturated steroids, triterpenes, saponins, flavonoids alkaloids and anthracene. The Gas Chromatography Mass Spectrometry of chloroform fraction of the extract revealed the presence of the following bioactive compounds: 4H-Pyran- 4-one, Iberin nitrile, 9-Borabicyclo[4.2.1]nonane, dimer, 3-Formylhept-3-enedinitrile, Pyridine, Palmitic acid, Pentadecanoic acid, 13-Tetradece-11-yn-1-ol, Decane, 3-chloro-3-Chlorodecane, 5,8-Octadecadienoic acid, E-9-Tetradecenoic acid, n-Hexadecanoic acid, Heptadecane, 11-Tridecen-1-ol, 3,11-Tetradecadien-1-ol and n-Pentadecane. The acute toxicity test of the extracts showed no sign of toxicity in the rats. One- way analysis of Variance was used to determine the significant differences of the extracts using SPSS statistical program. There was an increase in the parasitaemia, body temperature, white blood cell count, plasma protein while there was a decrease in the packed cell volume and red blood cell count in the infected rats. There was an increase in the weights of the organs in the infected animals. Necrosis in the testis, lymphocyte hyperplasia in the liver, kidney, spleen and vacoulations in the brain were observed. Therefore, the methanolic extract of the plant was more effective than the aqueous extract both in vivo and in vitro which completely cleared the parasites at the dose of 800mg/kg body weight on day 10.