BACTERIOLOGICAL AND SEROLOGICAL STUDIES OF BOVINE BRUCELLOSIS IN OBUDU CATTLE RANCH, CROSS RIVER STATE, NIGERIA
BACTERIOLOGICAL AND SEROLOGICAL STUDIES OF BOVINE BRUCELLOSIS IN OBUDU CATTLE RANCH, CROSS RIVER STATE, NIGERIA
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Date
2013-01
Authors
MAURICE, Nanven Abraham
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Abstract
Brucellosis is one of the most important zoonotic diseases worldwide particularly in
developing countries and it is endemic in Nigeria. The study is to detect Brucella organism
by serology and cultural methods in Obudu Cattle Ranch. Fourteen vaginal swab from
cows that aborted and a hygroma fluid from a bull were collected and cultured for Brucella.
A total of 354 cattle sera were examined for Brucella antibodies using Rose Bengal Plate
Test (RBPT), Serum Agglutination Test (SAT), 2-Mercaptoethanol (2-ME) and
Competitive Enzyme Linked Immunosorbent Assay (c-ELISA). Out of the 354 sera
examined 149(42.09%), 142(40.11%), 132(37.29%) and 100(28.25%) were positive for
Brucella antibodies using RBPT, SAT, 2-ME and c-ELISA respectively. Of the 113 males
examined 40(35.40%), 39(34.51%), 36(31.86%) and 27(23.89%) of the males were
positive while 109(45.23%), 142(58.92%), 85(35.27%) and 73(30.29%) of the 241 females
were positive to Brucella antibodies using RRBPT, SAT, 2-ME, and c-ELISA respectively.
There was however, no statistical significant association (P>0.05) between sexes. A total of
194 of the cattle examined were >5 years of age, 110 were of 2-5 years and 50 were of < 2
years. The cattle < 2 years of age had 10(20.00%), 9(18.00%), 6(12.00%) and 5(10.00%),
while age group 2-5 years of age had 44(40.00%), 42(38.18%), 28(25.42%) and
24(21.82%), and the age groups >5 years showed that 95(48.97%), 91(46.91%),
87(44.85%) and 71(36.60%) were all positive to Brucella antibodies using RBPT, SAT, 2ME
and c-ELISA respectively. There was a statistically significant association (P<0.05) in
prevalence rate between age groups with animals in the age group >5years. Breed-Specific
prevalence rate showed that of the 117 Kuri examined 73(62.39%), 69(58.97%),
62(52.99%) and 35(29.91%) and of the 131 Friesian examined 10(7.63%), 10(7.63%),
9(6.87%) and 9(6.87%) and of the 106 of White Fulani examined 66(62.26%), 63(59.43%),
51(48.11%) and 56(52.83%) of breeds were positive for Brucella antibodies using RBPT,
SAT, 2-ME and c-ELISA test respectively. There was a statistically significant association
(P<0.05) between prevalence rate in breeds examined. There was also high level of
correlation (0.7589) between the results obtained using RBPT, SAT and c-ELISA test. In
conclusion Brucella abortus biotype 1 was isolated and the serology status as it relates to
brucellosis has been documented, further studies will be carried out to characterize the
Brucella organism.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILMENT FOR THE REQUIRMENT FOR THE AWARD OF
MASTER OF SCIENCE IN VETERINARY MICROBIOLOGY
DEPARTMENT OF VETERINARY MICROBIOLOGY, AHMADU BELLO
UNIVERSITY, ZARIA, KADUNA STATE, NIGERIA
JANUARY, 2013
Keywords
BACTERIOLOGICAL,INE, SEROLOGICAL,, STUDIES,, BOVINE,, BRUCELLOSIS,, OBUDU,, CATTLE,, RANCH,, CROSS,, RIVER,, STATE,, NIGERIA