MOLECULARCHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATES FROM CHICKENSIN ZARIA AND ITS ENVIRONS, KADUNA STATE, NIGERIA
MOLECULARCHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATES FROM CHICKENSIN ZARIA AND ITS ENVIRONS, KADUNA STATE, NIGERIA
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Date
2016-05
Authors
HAMISU, Tasiu Mallam
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Abstract
Newcastle disease virus (NDV) has a negative-sense,single-strand RNA genome.Although all
NDVs are members of Avian Paramyxovirus-1 (APMV-1), antigenic and genetic diversity is
recognized. Consequently, different genotypes of APMV-1 circulate in different parts of the
world. Therefore, this study aimed to characterize circulating field NDV strains in Zaria and
identify their genotype/sub-genotype. A total of 127cloacal swabs were collected from local
chickens in live bird market and exotic chickens in commercial poultry farms in Zaria and
environs, Nigeria between November, 2014 and January, 2015. Five commercial poultry farms
and four live bird markets were purposively sampled.Molecular screening of NDV Matrix-gene
(M-gene) was performed on all the samples using ReverseTranscriptase-Polymerase Chain
Reaction (RT-PCR). Newcastle disease positive samples were further inoculated in to
embryonated chicken eggs for isolation of NDV. Isolates were confirmed as NDV by
haemaggulitination (HA) test and detection of the Fusion-g ene using RT- PCRT he partial F gene amplicons (containing nucleotide position 610 of NDV M-gene to position 581 of NDV Fgene)
were sequenced in Inqaba Biotechnology, South Africa and the results were analyzed
using BioEdit sequence analysis program and MEGA 6.6 software. Newcastle disease virus Mgene
were detected in 16 out of 127 cloacal swabs; 13 from live bird markets and 3 from
commercial poultry farms. However, only 10 NDVs were isolated in embryonated chicken eggs
as confirmed by HA and RT-PCR. Eight of the 10 amplicons of the partial F-gene were
successfully sequenced. Analysis of the 47-419 nucleotide region of the partial F-gene of these
sequences revealed that all the NDVs isolated in this study were virulent and based on the
bootstrap value of>60% and the tree topology, all the strains belonged to sub-genotype XIVb.
Three strains from this study shared common amino acid mutations P→L10 and A→V11 as therepresentatives of sub-genotype XIVb. In addition, one strain from this study sharedL→P36
common amino acid mutation with two representative strains of sub-genotype XIVb from
Nigeria and Benin Republic. However, when compared with two representatives of sub-genotype
XIVb, all strains from this study had R→Q114 mutations. This study therefore, highlights the
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES,
AHMADU BELLO UNIVERSITY, ZARIA
IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF THE
DEGREE OF MASTER OF SCIENCE IN VETERINARY MICROBIOLOGY
DEPARTMENT OF VETERINARY MICROBIOLOGY,
AHMADU BELLO UNIVERSITY, ZARIA NIGERIA
Keywords
MOLECULAR CHARACTERIZATION,, NEWCASTLE DISEASE VIRUS ISOLATES,, CHICKENSIN ZARIA,, ENVIRONS,, KADUNA STATE,, NIGERIA