Influence of Desiccation Time on Survival and Regeneration of Embryonic Axes of Groundnut {Arachis hypogaea L.) Immersed in Liquid Nitrogen
Influence of Desiccation Time on Survival and Regeneration of Embryonic Axes of Groundnut {Arachis hypogaea L.) Immersed in Liquid Nitrogen
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Date
2013-08
Authors
Ahdulmalik, M. M.
Usman, I. S.
Olarewaju, J. D.
Aba, D. A.
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Abstract
Cryoprcscrvation, the storage of biological materials in liquid nitrogen (LN), is a useful method for long term conservation of plant germplasin. This study was carried out with the objective of establishing an efficient desiccation technique for successful cryopreservalion and recovery of embryonic axes of groundnut. Rmbryonic axes of four groundnut {Arachis hypugaeu I..) genotypes were evaluated. The excised embryonic axes were dehydrated by air current of a laminar air flow cabinet for different duration (0, I, 2, 3, 4 & 5 hrs) before being plunged in LN (-196°C) and held for 1 hr. Samples were thawed in water bath at 40°C for 2 min, thereafter cultured on MS medium supplemented with 15 mg/L BAP for recovery. Highest survival (96.67% - 100%) and shoot formation (91..67% - 96.67%) were obtained at an average moisture content of 17% after 4 - 5 hr desiccation. Among the genotypes evaluated, Sannut 22 and Sanimit 23 recorded the highest survival and shoot formation, This technique therefore appears promising for cryoprcscrvation of groundnut gennplasm.
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Keywords
Cryoprcscrvation,, Groundnut,, Hmbryonie Axes,, Desiccation,