COMPARATIVE EVALUATION OF SOYBEAN (Glycine max L) EXTRACTS AND OESTROGEN CREAM ON EXPERIMENTAL CUTANEOUS WOUND HEALING IN OVARIECTOMIZED ALBINO RATS
COMPARATIVE EVALUATION OF SOYBEAN (Glycine max L) EXTRACTS AND OESTROGEN CREAM ON EXPERIMENTAL CUTANEOUS WOUND HEALING IN OVARIECTOMIZED ALBINO RATS
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Date
2018-11
Authors
OYENEKAN, Iskiil Oladehinde
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Abstract
This study was carried out to evaluate the comparative effects of topical soybeans extracts and oestrogen cream on cutaneous wound healing using excision wound model in ovariectomized Wistar albino rats. Twenty four (24) female Wistar albino rats of age 20 weeks weighing 200-250g were ovariectomised two weeks post acclimatisation and monitored for four weeks post-surgery for proper recovery. They were randomly divided into four groups (A, B, C, D) with 6 rats in each group. Two full-thickness skin excision measuring 1.5 x 1.5cm was surgically created at the dorsum of the thoraco-abdominal region of each rat. Wounds in all groups (A, B, C, D) were monitored and treated topically with normal saline, oestrogen cream, soybean crude-ethanolic extract and Soybean-chloroform extract respectively, for 20 days. Wound contraction was evaluated on day 4, 8 and 12 by wound tracing method after removing the scab while granulation tissue was harvested for histopathology, antioxidant assay, Marker of Oxidative Stress (Malondialdehyde (MDA)) and B-cell lymphoma 2 (Bcl-2) antiapoptotic protein assay on day 4, 8, 12 and 16. There was no significant (p>0.05) difference in the rate of wound contraction in the oestrogen cream (90.29 ± 0.75%) and soybean ethanolic extract (89.28 ± 0.95%) treated groups but both had a significant (p < 0.05) rate of wound contraction compared to normal saline treated group (64.56 ± 0.70%). Histopathology revealed that oestrogen cream and soybean ethanolic extract promoted formation of healthy granulation tissue, collagen deposition, fewer and regulated inflammatory cells infiltration, fibroblast proliferation and formation of new blood vessels. The wounds in normal saline, oestrogen cream, soybean crude-ethanolic extract and Soybean-chloroform extract treated groups healed on day 18.25 ± 0.25, 14.25 ± 0.25, 14.00 ± 0.00 and 16.25 ± 0.25 respectively.
Oestrogen cream and soybean ethanolic extract treated group had a significant (p < 0.001) increase in Reduced Glutathione (GSH) level (84.67 ± 2.34μg/ml, 82.27 ± 1.63 μg/ml respectively) on day 8 post wounding compared to normal saline (56.9 ± 8.09 μg/ml) treated group. Malondialdehyde (MDA) level reduced significantly (p < 0.001) on day 4 post wounding in oestrogen cream (77.37 ± 7.88 μmols/mgproteins), soybean ethanolic extract (90.13 ± 3.32 μmols/mgproteins), and soybean chloroform extract (86.60 ± 6.99 μmols/mgproteins) treated group compared to normal saline (180.97 ± 10.02 μmols/mgproteins). Also, there was significant (p < 0.05) increased expression of Bcl-2 antiapoptotic protein on day 4 and 8 of wound healing in the oestrogen cream with quick score (10.00 ± 1.00, 11.00 ± 1.00 respectively) and soybean ethanolic extract with quick score (8.00 ± 1.00, 10.00 ± 1.00 respectively) compared to normal saline control group with quick score (5.33 ± 0.67, 6.33 ± 1.45 respectively) indicating reduced cellular apoptosis during the proliferative phase of wound healing. In conclusion, this study demonstrated that topical treatment of cutaneous wound with soybean ethanolic extract had comparable efficacy to oestrogen in the management of wound in ovariectomized wistar albino rats.
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A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN VETERINARY SOFT TISSUE SURGERY DEPARTMENT OF VETERINARY SURGERY AND RADIOLOGY, AHMADU BELLO UNIVERSITY, ZARIA
Keywords
COMPARATIVE EVALUATION,, SOYBEAN,, EXTRACTS,, OESTROGEN CREAM,, EXPERIMENTAL CUTANEOUS WOUND HEALING,, OVARIECTOMIZED ALBINO RATS