EVALUATION OF THE EFFECT OF VITAMINS C AND E ON SPERMIOGRAM AND MALONDIALDEHYDE CONCENTRATION IN CHILLED SEMEN OF NIGERIAN INDIGENOUS TURKEY TOMS (Meleagris gallopavo)
EVALUATION OF THE EFFECT OF VITAMINS C AND E ON SPERMIOGRAM AND MALONDIALDEHYDE CONCENTRATION IN CHILLED SEMEN OF NIGERIAN INDIGENOUS TURKEY TOMS (Meleagris gallopavo)
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Date
2016-03
Authors
ECHEKWU, WILSON OCHIFE
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Abstract
Oxidative stress has been established as a major cause of semen deterioration during in-vitro storage. The aim of this study was to evaluate the effects of supplementing the diluents of turkey semen with antioxidant vitamins C, E and their combination on spermiogram and lipid peroxidation during in-vitro storage at 40C. Twenty healthy turkey toms aged 37-38 weeks with an average weight of 7±0.3kg were used for this study. The toms were randomly placed into four groups (T0, T1, T2 and T3) of five toms. Semen was collected bi-weekly for 8 weeks from the turkey toms within the groups using abdominal massage method. Following collection, the initial semen characteristics and Malondialdehyde concentration were evaluated. Semen collected from individual turkey toms within each group were pooled, evaluated and extended with egg yolk citrate diluent containing no antioxidant (T0), 4 mg/ml Vitamin C (T1), 8 mg/ml Vitamin E (T2) and 4 mg/ml Vitamin C + 8 mg/ml Vitamin E (T3). The extended semen were packaged into bijju bottles and stored at 40C for 48 hours. Semen motility, viability, morphology and Malondialdehyde concentration were assessed at 0, 3, 6, 9, 12, 24 and 48 hours post dilution and storage at 40C. The motility and viability of the diluted semen decreased across the group with increase in storage time irrespective of the antioxidants added. T2 gave a better motility (55±2.8 %), viability (41.3± 1.5%) and lower morphological defects (24.3±0.3%) over a 24 hour period as compared to T1(0.0 ± 0.0 %, 19.0 ± 3.4 % and 30.3 ± 0.9%, respectively) and T3 (10.0 ± 5.7%, 25.0 ± 4.0% and 26.7 ± 0.3 %, respectively). The poorest motility, viability and morphological defect results at 24 hours of storage, were obtained in the control (0.0 ± 0.0 %, 10.3 ± 1.2 % and 42.7 ± 2.3%, respectively). Malondialdehyde concentration increased with storage time across the group irrespective of the antioxidants supplemented. However, T2 had a lower Malondialdehyde concentration (2.5 ± 0.2μl/ml) at 24 hour of storage as compared to T0 (3.1 ± 0.2 μl/ml), T1(2.7 ± 0.2 μl/ml) and T3(2.1 ± 0.2 μl/ml). The result from this study showed that supplementation of the diluents of turkey semen with antioxidants resulted in significant (p <0.05) improvement in spermatozoa motility, viability and a reduced rate of lipid peroxidation compared to the control (T0). Vitamin E performed better than vitamin C and a combination of vitamin C+E in maintaining turkey semen motility (55.0 ± 2.9 %), viability (41.3 ±1.4 %), lower rate of morphological defects (24.2± 0.3 %) and a lower MDA level (2.5±0.2) after 24 hour of storage at 40C. It was concluded that the supplementation of diluents of turkey semen with Vitamin E or a combination of vitamin C and E could preserve turkey semen for 24 hours at 40C.
Description
A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A DEGREE OF MASTER OF SCIENCE IN THERIOGENOLOGY DEPARTMENT OF THERIOGENOLOGY AND PRODUCTION, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
EVALUATION,, VITAMINS C AND E ON SPERMIOGRAM,, MALONDIALDEHYDE CONCENTRATION, CHILLED SEMEN,, NIGERIAN,, TURKEY TOMS,, Meleagris gallopavo,