CHARACTERIZATION OF CAMPYLOBACTER SPECIES ISOLATED FROM SHEEP AND EFFECTS OF CRUDE EXTRACTS OF ACACIA NILOTCA AND VITEXDONlANA ON THE ISOLATES
CHARACTERIZATION OF CAMPYLOBACTER SPECIES ISOLATED FROM SHEEP AND EFFECTS OF CRUDE EXTRACTS OF ACACIA NILOTCA AND VITEXDONlANA ON THE ISOLATES
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Date
1998-12
Authors
RAJI, Mashood Abiola
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Abstract
Campylobacter species isolated from Kaduna State were characterized into their species
and bioserogrouped. Their susceptibilites to the extract of the leaves of Acacia nilotica bark of
Vitex doniana were studied. A total of 1100 sheep samples were collected from Kaduna abattoir
and National Animal Production Reach Institute (NAPRI), Shika. The samples comprised 250
gallbladder, 250 intestinal contents, 100 fetal stomach contents from Kaduna abattoir while 250
rectal swabs and 250 vaginal swabs were obtained from sheep at the NAPRI Small Ruminant
Programme flocks.
Of the total of 1100 samples, 39(3.54%) yielded Campylobacter organisms. The highest
isolation rate (6.8%) was from samples of intestinal contents followed by those from gall
bladdcrs(4.0%). Samples from the vaginal swabs and fetuses had the lowest isolation rates of
2.80% and 0% respectively.
Of all the 39 Campylobacter isolates, 79% were characterized as C. fetus subsp jejuni,
13% as c. coli, and 8.0% as C. laridis- Campylohacter coli and C. laridis were isolated from
gall bladder and intestinal contents only but not from vaginal and rectal swabs. Campylobacter
jejuni biotype 1 accounted for 40.3% of the total isolates, C laridis biotypes 1 and 11 were also
isolated and accounted for 5 % and 3 % of the isolates respectively and 5% of the isolates were nontypcable
Serogroups 36 and 4 were the commonest serogroups repesenting 10.3% and 7.7%of all
serogroups respectively. Other serogroups recorded in sheep in Kaduna State were 29
(5.1%),20(7.69%) and 55 (2.6%). They all belonged to C. jejuni Campylobacter coli were
represented by serogroups 4 (7.7%) 6 and 84 repesenting 2.56% each, C .laridis isolates were not
serotyped. There was no significant difference in the recovery of the different serogroups from
the different specimens investigated in sheep at two locations surveyed.
The minimum inhibitory concentration of A nilotica ethanol extract was 80 mg/ ml while
that of the water extract for Acacia nolitica was 250 mg/ ml. Both the water and ethanol extracts of
the V doniana had no inhibitory effect on the Campylobacter isolates at concentrations up to 500
mg/ml.
The isolation of Campylobacter organisms from rectum, vagina, gallbladder, and intestinal
contents is a clear indication that sheep may serve as a reservior of these organisms in
Nigeria.Similarities between documented human Campylobacter isolates in Nigeria and those in the
present study raise the possibility of cross-transmission between sheep and man. It is concluded that
biotyping and serogrouping can be used for epidemiological study of campylobacteriosis due to
Campylobacter jejuni in sheep in Kaduna State of Nigeria.
Description
A thesis submitted to the Postgraduate School,
Ahmadu Bcllo University, Zaria,
in partial fulfillment of the requirement for the
Award of Degree of Master of Science.
Department of Veterinary Pathology and Microbiology,
faculty of Veterinary Medicine,
Ahmadu Bello University, Zaria
DECEMBER, 1998
Keywords
CHARACTERIZATION,, CAMPYLOBACTER,, SPECIES,, ISOLATED,, SHEEP,, EFFECTS,, CRUDE,, EXTRACTS,, ACACIA,, NILOTCA,, VITEXDONlANA,, ISOLATES.