ISOLATION OF MYCOPLASMA BOVIS AND DETECTION OF ITS ANTIBODIES IN CATTLE IN THREE LOCAL GOVERNMENT AREAS OF ADAMAWA STATE, NIGERIA
ISOLATION OF MYCOPLASMA BOVIS AND DETECTION OF ITS ANTIBODIES IN CATTLE IN THREE LOCAL GOVERNMENT AREAS OF ADAMAWA STATE, NIGERIA
No Thumbnail Available
Date
2014-04
Authors
FRANCIS, MARKUS ISA
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Mycoplasma bovis is an important pathogen causing pneumonia, mastitis, arthritis and variety of other diseases in cattle worldwide. Pneumonic lungs samples were cultured and recovered isolates were biochemically identified for M. bovis. At the same time serum samples were collected and tested for the presence of antibodies to M. bovis using ELISA Kit (Bio-X Diagnostics, Jemelle-Belgium). A total of four hundred (400) serum samples and one hundred (100) pneumonic lungs were collected from three Local Government Areas in Adamawa State. A prevalence of M. bovis of 2.0% (2/100) was obtained from culture of pneumonic lungs. The isolates were observed to reduced tetrazolium chloride and had phosphatase activity; neither do they hydrolyzed glucose nor catabolized arginine. An overall seroprevalence of M. bovis detected by ELISA was 19.5% (78/400). The highest seroprevalence was recorded in Ganye LGA 36 (27.7%), followed by Yola LGA 28 (20.0%) and lowest was Mubi LGA 14 (10.8%), the association is statistically significant (p<0.05). Age- specific seroprevalence showed that cattle less than one-year old had the highest prevalence (25.8%), followed by four year-old cattle (22.4%). Based on sex distribution, 20.4% of the females and 17.4% of the males were seropositive to M. bovis. Breed susceptibility to M. bovis infection showed that White Fulani (21.8%) had the highest prevalence, followed by Cross breed (18.5%), Red Bororo (16.4%) and Adamawa Gudali (11.9%). There was no statistical significant association found among M. bovis infection and age, sex and breed distribution (p>0.05). The isolation as well as seroprevalence of M. bovis showed that this organism can have a serious effect on both calves and adult cattle, although the true extent of the disease is often overlooked. In conclusion, this study suggests that M. bovis is endemic in the study area and the culture and serology are important in complementing each other in the diagnosis. There is need for regular routine serological
surveillance that will cover more Local Government Areas to ascertain the prevalence of the disease in the study area.
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN VETERINARY MICROBIOLOGY DEPARTMENT OF VETERINARY MICROBIOLOGY, FACULTY OF VETERINARY MEDICINE, AHMADU BELLO UNIVERSITY, ZARIA NIGERIA
APRIL, 2014
Keywords
ISOLATION,, MYCOPLASMA,, BOVIS,, ANTIBODIES,, CATTLE,, LOCAL GOVERNMENT AREAS,, ADAMAWA STATE, NIGERIA